Overview

  • Product name

    Anti-LRMP antibody [EPR17852]
    See all LRMP primary antibodies
  • Description

    Rabbit monoclonal [EPR17852] to LRMP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human LRMP aa 1-250. The exact sequence is proprietary.
    Database link: Q12912

  • Positive control

    • WB: Daudi, Ramos and NAMALWA cell lysates; Human tonsil lysate. IHC-P: Human tonsil tissue. ICC/IF: Jurkat and Ramos cells. FC: Jurkat
  • General notes

     

     

     This product was previously labelled as Jaw1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17852
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab202418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/300.
Flow Cyt 1/600.

Target

  • Tissue specificity

    Expressed at high levels in pre B-cells, mature B-cells and pre T-cells. Expressed at low levels in mature T-cells and plasma B-cells.
  • Post-translational
    modifications

    The removal of the C-terminal lumenal domain occurs by proteolytic processing.
  • Cellular localization

    Cytoplasm and Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Lrmp antibody
    • LRMP_HUMAN antibody
    • Lymphoid restricted membrane protein antibody
    • Processed lymphoid-restricted membrane protein antibody
    • Protein Jaw1 antibody
    see all

Images

  • All lanes : Anti-LRMP antibody [EPR17852] (ab202418) at 1/10000 dilution

    Lane 1 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysate
    Lane 2 : NAMALWA (Human Burkitt's lymphoma) whole cell lysate
    Lane 3 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 62 kDa
    Observed band size: 62 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling LRMP with ab202418 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human tonail tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Ramos (Human Burkitt's lymphoma cell line) cells labeling LRMP with ab202418 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on Ramos cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202418 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • All lanes : Anti-LRMP antibody [EPR17852] (ab202418) at 1/10000 dilution

    Lane 1 : Human tonsil lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal heart lysate
    Lane 4 : Human fetal kidney lysate
    Lane 5 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 62 kDa
    Observed band size: 62 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling LRMP with ab202418 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202418 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Flow cytometry analysis of Jurkat cells labelling LRMP (red) with purified ab202418 at dilution of 1/600. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody used was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

References

ab202418 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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