Overview

  • Product name
    Anti-LRP1 antibody [8G1]
    See all LRP1 primary antibodies
  • Description
    Mouse monoclonal [8G1] to LRP1
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, WB, ICC/IF, Electron Microscopy, Dot blot, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to LRP1. J Biol Chem 265:17401-4 (1990).

Applications

Our Abpromise guarantee covers the use of ab20384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 1 - 10 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Use under non reducing condition.
ICC/IF Use a concentration of 5 µg/ml.
Electron Microscopy Use at an assay dependent concentration. Use at dilutions of 1/400 or 1/600 for 20 mins.
Dot blot Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.
    Functions as a receptor for Pseudomonas aeruginosa exotoxin A.
  • Tissue specificity
    Most abundant in liver, brain and lung.
  • Sequence similarities
    Belongs to the LDLR family.
    Contains 22 EGF-like domains.
    Contains 31 LDL-receptor class A domains.
    Contains 34 LDL-receptor class B repeats.
  • Post-translational
    modifications
    Cleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane.
    The N-terminus is blocked.
    Phosphorylated on serine and threonine residues.
    Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1.
  • Cellular localization
    Cytoplasm. Nucleus. After cleavage, the intracellular domain (LRPICD) is detected both in the cytoplasm and in the nucleus and Cell membrane. Membrane, coated pit.
  • Information by UniProt
  • Database links
  • Alternative names
    • A2MR antibody
    • Alpha 2 macroglobulin receptor antibody
    • alpha 2MR antibody
    • Alpha-2-macroglobulin receptor antibody
    • APOER antibody
    • Apolipoprotein E receptor antibody
    • APR antibody
    • CD 91 antibody
    • CD91 antibody
    • CD91 antigen antibody
    • IGFBP3R antibody
    • LDL receptor related protein 1 antibody
    • Low density lipoprotein receptor related protein 1 antibody
    • Low density lipoprotein related protein 1 antibody
    • Low-density lipoprotein receptor-related protein 1 intracellular domain antibody
    • LRP 1 antibody
    • LRP 515 antibody
    • LRP 85 antibody
    • LRP antibody
    • LRP ICD antibody
    • LRP-1 antibody
    • LRP-515 antibody
    • LRP-85 antibody
    • Lrp1 antibody
    • LRP1 protein antibody
    • LRP1_HUMAN antibody
    • LRP1A antibody
    • LRP515 antibody
    • LRP85 antibody
    • LRPICD antibody
    • MGC88725 antibody
    • Prolow density lipoprotein receptor related protein 1 antibody
    • TbetaR V/LRP 1/IGFBP 3 receptor antibody
    • TbetaRV/LRP1/IGFBP3 receptor antibody
    • TGFBR 5 antibody
    • TGFBR5 antibody
    • Type V tgf beta receptor antibody
    see all

Images

  • ICC/IF image of ab20384 stained Mcf7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20384, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab20384 staining human mesothelial cells by flow cytometry.  Cells were lifted in lifting buffer plus FBS and fixed with formaldehyde.  The cell population was gated against mIgG control.  The primary antibody was diluted 1/100 in 0.2% BSA in PBS and incubated with the sample at a concentration of 1 µg/100 µl for 1 hour at 4°C.  A FITC conjugated goat anti-mouse antibody was used as the secondary, diluted 1/100 and incubated with the sample at a concentration of 1 µg/100 µl.

    See Abreview

  • Ab20384 staining primary rat hepatocytes cells by EM. The cells were fixed and prepared by Tokuyasu method. The cryosections of 70nm were placed on 100 mesh grids. All the following steps were done at room temperature. Blocking was carried out for 30 min with 1% fish skin gelatine (FSG). The tissue sections were placed on a drop of 5 ul of first Ab (anti-LRP 8G1) for 20 min at dilutions of 1:400 or 1:600 seemed to work the best. After incubation with the primary antibody, 3. 5 x 2 min wash was done with PBS and the sections were placed on a drop of 5 ul of secondary Ab (goat anti-mouse) for 20 min (dilution 1:150 5. 5 x 2 min wash with PBS). Samples were placed on a drop of 5 ul of PAG10 for 15 min (dilution 1:60 or 1:70) and then 5 x 2 min wash with PBS. 2 min on 1% glutaraldehyde for fixation and 4 x 2.5 min on H2O. afterthat 1 second + 1 second + 6 min on drops of Methyl Cellulose/Uranyl Acetate (9:1) on ice. The surplus of MC/UA was removed , the slides were dried and obser

References

This product has been referenced in:
  • Boyé K  et al. The role of CXCR3/LRP1 cross-talk in the invasion of primary brain tumors. Nat Commun 8:1571 (2017). Read more (PubMed: 29146996) »
  • Chanalaris A  et al. Suramin Inhibits Osteoarthritic Cartilage Degradation by Increasing Extracellular Levels of Chondroprotective Tissue Inhibitor of Metalloproteinases 3. Mol Pharmacol 92:459-468 (2017). Read more (PubMed: 28798097) »
See all 10 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (hepatocyte)
Permeabilization
No
Specification
hepatocyte
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 10 2018

Answer

I checked the LRP1 antibodies and ab20384 has 93% homology with C. elegans.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

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Answer

Thank you for confirming this information and for your help and cooperation with this case.

As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order.

Credit ID: ####


As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. Please refer to the credit ID number in any correspondence with the accounting department.

I would like to wish the customer good luck with their research. The technical team is always at your service, should you require further expert advice.

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Answer

Thank you for taking the time to provide the further information.

The details you have kindly provided will provide us with vital information for our monitoring of product quality

I appreciate the time the customer has spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results. I can suggest you have regrettably received a bad vial. I apologize for the inconvenience.

I have also spoken to our distributors team and I am happy to let you know we are prepared to make an exception and continue to provide our Abpromise for Symbios customers. Therefore, I am pleased to offer you a free of charge replacement or credit note in compensation for this case.

In addition, I can suggest the customer may like to consider the following suggestions as a check for future experiments:

1. I can suggest to try adding 0.2% Tween to the antibody dilution buffer and wash buffer. This will help to solubilise the antibody and keep the cells permeabilised.

2. I can recommend to try wash steps: 3 X 5 minutes in PBS containing 0.2% Tween.

3. We recommend to incubate at 4oC overnight with antibodies. This can often provide more efficient and specific staining.

4. Is the current vial of secondary antibody working well with other primary antibodies?

I hope this will be helpful. I look forward to hearing from you with details of how you would like to proceed.

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Question
Answer

Thank you for contacting us.

For ab20384, the lab recommends using human umbilical vein endothelial cells (HUVEC) as a negative control. If you do not have access to this type of cells, according to the human protein atlas (linked below),Low Density LRP is not expressed in tonsil, skin, or breast tissues, so any of these could be used as a negative control. If you are looking for lysates, you could try ab30090, ab30166, or ab29614 and forslides, you could try ab4365.

http://www.proteinatlas.org/ENSG00000123384/normal

I hope this helps, please let me know if you need any additional information.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Mesothelial Cels)
Specification
Mesothelial Cels
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
gated against mIgG control

Abcam user community

Verified customer

Submitted Apr 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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