

Anti-LRP1 antibody [EPR3724] (ab92544)
- Datasheet
- References (66)
- Protocols
Overview
-
Product nameAnti-LRP1 antibody [EPR3724]
See all LRP1 primary antibodies -
DescriptionRabbit monoclonal [EPR3724] to LRP1
-
Host speciesRabbit
-
Tested applicationsSuitable for: IHC-P, WB, IP, Flow Cyt, ICC/IF, IHC-Frmore details
-
Species reactivityReacts with: Mouse, Rat, Human, Pig
-
Immunogen
Synthetic peptide within Human LRP1 aa 4450 to the C-terminus. The exact sequence is proprietary.
-
Positive control
- WB: PMBC and A549 cell lysates; mouse brain, heart, kidney and spleen tissue lysates; rat brain, heart, kidney or spleen tissue lysates; human fetal brain tissue lysates; pig liver and heart tissue lysates. IHC-P: Human liver, clear cell carcinoma, brain, lung and placenta tissues. ICC/IF: U87-MG cells. Flow Cyt: Jurkat cells. IP: A549 cells.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
-
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
PurityProtein A purified
-
ClonalityMonoclonal
-
Clone numberEPR3724
-
IsotypeIgG
-
Research areas
Associated products
-
Alternative Products
- Anti-LRP1 antibody [EPR3724] (Alexa Fluor® 488) (ab195567)
- Anti-LRP1 antibody [EPR3724] (Alexa Fluor® 647) (ab195568)
- Anti-LRP1 antibody [EPR3724] (Alexa Fluor® 594) (ab206902)
- Anti-LRP1 antibody [EPR3724] - BSA and Azide free (ab215997)
- Anti-LRP1 antibody [EPR3724] (Allophycocyanin) (ab225059)
- Anti-LRP1 antibody [EPR3724] (Phycoerythrin) (ab225060)
- Anti-LRP1 antibody [5A6] (ab28320)
-
Alternative Versions
- Anti-LRP1 antibody [EPR3724] (Alexa Fluor® 488) (ab195567)
- Anti-LRP1 antibody [EPR3724] (Alexa Fluor® 647) (ab195568)
- Anti-LRP1 antibody [EPR3724] (HRP) (ab195569)
- Anti-LRP1 antibody [EPR3724] (Alexa Fluor® 594) (ab206902)
- Anti-LRP1 antibody [EPR3724] (Alexa Fluor® 555) (ab206904)
- Anti-LRP1 antibody [EPR3724] - BSA and Azide free (ab215997)
- Anti-LRP1 antibody [EPR3724] (Allophycocyanin) (ab225059)
- Anti-LRP1 antibody [EPR3724] (Phycoerythrin) (ab225060)
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab92544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/5000 - 1/10000. |
|
WB | 1/50000. Predicted molecular weight: 85 kDa. For unpurified use at 1/20000 - 1/50000. |
|
IP | 1/30. For unpurified use at 1/50. |
|
Flow Cyt | 1/100. For unpurified use at 1/1000. |
|
ICC/IF | 1/100. For unpurified use at 1/5000 - 1/10000. |
|
IHC-Fr | Use at an assay dependent concentration. |
Target
-
FunctionEndocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.
Functions as a receptor for Pseudomonas aeruginosa exotoxin A. -
Tissue specificityMost abundant in liver, brain and lung.
-
Sequence similaritiesBelongs to the LDLR family.
Contains 22 EGF-like domains.
Contains 31 LDL-receptor class A domains.
Contains 34 LDL-receptor class B repeats. -
Post-translational
modificationsCleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane.
The N-terminus is blocked.
Phosphorylated on serine and threonine residues.
Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1. -
Cellular localizationCytoplasm. Nucleus. After cleavage, the intracellular domain (LRPICD) is detected both in the cytoplasm and in the nucleus and Cell membrane. Membrane, coated pit.
- Information by UniProt
-
Database links
- Entrez Gene: 4035 Human
- Entrez Gene: 16971 Mouse
- Entrez Gene: 299858 Rat
- Omim: 107770 Human
- SwissProt: Q07954 Human
- SwissProt: Q91ZX7 Mouse
- Unigene: 162757 Human
- Unigene: 271854 Mouse
see all -
Alternative names
- A2MR antibody
- Alpha 2 macroglobulin receptor antibody
- alpha 2MR antibody
see all
Images
-
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: LRP1 knockout HAP1 cell lysate (20 µg)
Lane 3: A549 cell lysate (20 µg)
Lane 4: Mouse heart tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab92544 observed at 92 kDa. Red - loading control, ab8245, observed at 37 kDa.ab92544 was shown to specifically react with LRP1 in wild-type HAP1 cells. No band was observed when LRP1 knockout samples were used. Wild-type and LRP1 knockout samples were subjected to SDS-PAGE. ab92544 and ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
-
Immunocytochemistry/Immunofluorescence analysis of U87-MG cells labelling LRP1 with purified ab92544 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRP1 antibody [EPR3724] (ab92544)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human clear cell carcinoma of the kidney tissue labelling LRP1 with purified ab92544 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
-
Flow Cytometry analysis of Jurkat cells labelling LRP1 with purified ab92544 at 1/100 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
-
All lanes : Anti-LRP1 antibody [EPR3724] (ab92544) at 1/50000 dilution (purified)
Lane 1 : A549 cell lysate
Lane 2 : Human fetal brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
ab92544 (purified) at 1/30 immunoprecipitating LRP1 in A549 cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
All lanes : Anti-LRP1 antibody [EPR3724] (ab92544) at 1/50000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRP1 antibody [EPR3724] (ab92544)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human brain tissue labelling LRP1 with unpurified ab92544.
-
Immunocytochemistry/ Immunofluorescence - Anti-LRP1 antibody [EPR3724] (ab92544)This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
ICC/IF image of LRP1 staining on rat mixed glia culture using unpurified ab92544 (1:200). The cells were fixed using paraformaldehyde. The cells were then permeabilised using 0.1% TritonX in 0.1% PBS. Non-specific protein was blocked using 10% donkey serum at 24°C for 1 hour. ab92544 was diluted (1/200) using 0.1% TritonX with 0.1% PBS and 10% donkey serum and the cells were incubated for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to Rabbit IgG conjugated to Alexa Fluor® 488. DAPI was used to stain the nucleus.
-
Overlay histogram showing Jurkat cells stained with unpurified ab92544 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92544, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
-
All lanes : Anti-LRP1 antibody [EPR3724] (ab92544) at 1/50000 dilution (purified)
Lane 1 : Pig liver tissue lysate
Lane 2 : Pig heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRP1 antibody [EPR3724] (ab92544)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human lung tissue labelling LRP1 with unpurified ab92544.
-
All lanes : Anti-LRP1 antibody [EPR3724] (ab92544) at 1/20000 dilution (unpurified)
Lane 1 : Human PMBC lysate
Lane 2 : A549 lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse heart lysate
Lane 5 : Mouse kidney lysate
Lane 6 : Mouse spleen lysate
Lane 7 : Rat brain lysate
Lane 8 : Rat heart lysate
Lane 9 : Rat kidney lysate
Lane 10 : Rat
spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRP1 antibody [EPR3724] (ab92544)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human placenta tissue labelling LRP1 with unpurified ab92544.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRP1 antibody [EPR3724] (ab92544)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling LRP1 with unpurified ab92544 at 1/100.
Protocols
References
This product has been referenced in:
- Chen L et al. PID1 in adipocytes modulates whole-body glucose homeostasis. Biochim Biophys Acta 1861:125-132 (2018). Read more (PubMed: 29391195) »
- Chea C et al. Molecular mechanism of inhibitory effects of bovine lactoferrin on the growth of oral squamous cell carcinoma. PLoS One 13:e0191683 (2018). Read more (PubMed: 29381751) »