Overview

  • Product name

    Anti-LRP1 antibody [EPR3724] (Allophycocyanin)
    See all LRP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3724] to LRP1 (Allophycocyanin)
  • Host species

    Rabbit
  • Conjugation

    Allophycocyanin. Ex: 645nm, Em: 660nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide within Human LRP1 aa 4450 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • Flow Cytometry: Neuro-2a cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab225059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.
    Functions as a receptor for Pseudomonas aeruginosa exotoxin A.
  • Tissue specificity

    Most abundant in liver, brain and lung.
  • Sequence similarities

    Belongs to the LDLR family.
    Contains 22 EGF-like domains.
    Contains 31 LDL-receptor class A domains.
    Contains 34 LDL-receptor class B repeats.
  • Post-translational
    modifications

    Cleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane.
    The N-terminus is blocked.
    Phosphorylated on serine and threonine residues.
    Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1.
  • Cellular localization

    Cytoplasm. Nucleus. After cleavage, the intracellular domain (LRPICD) is detected both in the cytoplasm and in the nucleus and Cell membrane. Membrane, coated pit.
  • Information by UniProt
  • Database links

  • Alternative names

    • A2MR antibody
    • Alpha 2 macroglobulin receptor antibody
    • alpha 2MR antibody
    • Alpha-2-macroglobulin receptor antibody
    • APOER antibody
    • Apolipoprotein E receptor antibody
    • APR antibody
    • CD 91 antibody
    • CD91 antibody
    • CD91 antigen antibody
    • IGFBP3R antibody
    • LDL receptor related protein 1 antibody
    • Low density lipoprotein receptor related protein 1 antibody
    • Low density lipoprotein related protein 1 antibody
    • Low-density lipoprotein receptor-related protein 1 intracellular domain antibody
    • LRP 1 antibody
    • LRP 515 antibody
    • LRP 85 antibody
    • LRP antibody
    • LRP ICD antibody
    • LRP-1 antibody
    • LRP-515 antibody
    • LRP-85 antibody
    • Lrp1 antibody
    • LRP1 protein antibody
    • LRP1_HUMAN antibody
    • LRP1A antibody
    • LRP515 antibody
    • LRP85 antibody
    • LRPICD antibody
    • MGC88725 antibody
    • Prolow density lipoprotein receptor related protein 1 antibody
    • TbetaR V/LRP 1/IGFBP 3 receptor antibody
    • TbetaRV/LRP1/IGFBP3 receptor antibody
    • TGFBR 5 antibody
    • TGFBR5 antibody
    • Type V tgf beta receptor antibody
    see all

Images

  • Overlay histogram showing Neuro-2a cells stained with ab225059 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225059, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

References

ab225059 has not yet been referenced specifically in any publications.

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