Key features and details
- Rabbit polyclonal to Lrp2 / Megalin
- Suitable for: IHC-P
- Reacts with: Mouse, Human, Monkey
- Isotype: IgG
Product nameAnti-Lrp2 / Megalin antibody
See all Lrp2 / Megalin primary antibodies
DescriptionRabbit polyclonal to Lrp2 / Megalin
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Mouse, Human, Monkey
Predicted to work with: Rat, Xenopus laevis
Synthetic peptide corresponding to Human Lrp2/ Megalin aa 4600 to the C-terminus (C terminal).
(Peptide available as
- This antibody gave a positive signal in Human Kidney Carcinoma Tissue (Immunohistochemistry).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab76969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionActs together with cubilin to mediate HDL endocytosis (By similarity). May participate in regulation of parathyroid-hormone and para-thyroid-hormone-related protein release.
Tissue specificityAbsorptive epithelia, including renal proximal tubules.
Involvement in diseaseDefects in LRP2 are the cause of Donnai-Barrow syndrome (DBS) [MIM:222448]; also known as faciooculoacousticorenal syndrome (FOAR syndrome). DBS is a rare autosomal recessive disorder characterized by major malformations including agenesis of the corpus callosum, congenital diaphragmatic hernia, facial dysmorphology, ocular anomalies, sensorineural hearing loss and developmental delay. The FOAR syndrome was first described as comprising facial anomalies, ocular anomalies, sensorineural hearing loss, and proteinuria. DBS and FOAR were first described as distinct disorders but the classic distinguishing features between the 2 disorders were presence of proteinuria and absence of diaphragmatic hernia and corpus callosum anomalies in FOAR. Early reports noted that the 2 disorders shared many phenotypic features and may be identical. Although there is variability in the expression of some features (e.g. agenesis of the corpus callosum and proteinuria), DBS and FOAR are now considered to represent the same entity.
Sequence similaritiesBelongs to the LDLR family.
Contains 17 EGF-like domains.
Contains 36 LDL-receptor class A domains.
Contains 37 LDL-receptor class B repeats.
Cellular localizationMembrane. Membrane > coated pit.
- Information by UniProt
- Calcium sensor protein antibody
- DBS antibody
- Glycoprotein 330 antibody
IHC image of Lrp2/Megalin antibody staining in a section of formalin-fixed paraffin-embedded human kidney renal carcinoma* performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab76969, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
ab76969 staining Lrp2 / Megalin in monkey kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate pH 6.0. Samples were then incubated with ab76969 at 1µg/ml for 1 hour with BSA in TBS. The secondary used was an undiluted HRP conjugated goat anti-rabbit polyclonal.
ab76969 staining Lrp2 / Megalin in mouse kidney tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, blocked for 60 minutes at 25°C and antigen retrieval was by heat mediation. The sample was incubated with primary antibody (1/1000) at 25°C for 1 hour. An Alexa Fluor® 488-conjugated donkey rabbit polyclonal (1/1200) was used as the secondary antibody.
ab76969 has been referenced in 16 publications.
- Kang HM et al. Effective reconstruction of functional organotypic kidney spheroid for in vitro nephrotoxicity studies. Sci Rep 9:17610 (2019). PubMed: 31772214
- Tian D et al. The effect of A1 adenosine receptor in diabetic megalin loss with caspase-1/IL18 signaling. Diabetes Metab Syndr Obes 12:1583-1596 (2019). PubMed: 31695457
- Rao C et al. MiR-219a-5p enhances cisplatin sensitivity of human non-small cell lung cancer by targeting FGF9. Biomed Pharmacother 114:108662 (2019). PubMed: 30999114
- Silva-Aguiar RP et al. O-GlcNAcylation reduces proximal tubule protein reabsorption and promotes proteinuria in spontaneously hypertensive rats. J Biol Chem 293:12749-12758 (2018). PubMed: 29954945
- Maydan O et al. Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy. Physiol Rep 6:e13654 (2018). Mouse . PubMed: 29595914