Overview

  • Product name

    Anti-Lrp2 / Megalin antibody
    See all Lrp2 / Megalin primary antibodies
  • Description

    Rabbit polyclonal to Lrp2 / Megalin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Monkey
    Predicted to work with: Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human Lrp2/ Megalin aa 4600 to the C-terminus (C terminal).
    (Peptide available as ab101417)

  • Positive control

    • This antibody gave a positive signal in Human Kidney Carcinoma Tissue (Immunohistochemistry).

Properties

Applications

Our Abpromise guarantee covers the use of ab76969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Acts together with cubilin to mediate HDL endocytosis (By similarity). May participate in regulation of parathyroid-hormone and para-thyroid-hormone-related protein release.
  • Tissue specificity

    Absorptive epithelia, including renal proximal tubules.
  • Involvement in disease

    Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS) [MIM:222448]; also known as faciooculoacousticorenal syndrome (FOAR syndrome). DBS is a rare autosomal recessive disorder characterized by major malformations including agenesis of the corpus callosum, congenital diaphragmatic hernia, facial dysmorphology, ocular anomalies, sensorineural hearing loss and developmental delay. The FOAR syndrome was first described as comprising facial anomalies, ocular anomalies, sensorineural hearing loss, and proteinuria. DBS and FOAR were first described as distinct disorders but the classic distinguishing features between the 2 disorders were presence of proteinuria and absence of diaphragmatic hernia and corpus callosum anomalies in FOAR. Early reports noted that the 2 disorders shared many phenotypic features and may be identical. Although there is variability in the expression of some features (e.g. agenesis of the corpus callosum and proteinuria), DBS and FOAR are now considered to represent the same entity.
  • Sequence similarities

    Belongs to the LDLR family.
    Contains 17 EGF-like domains.
    Contains 36 LDL-receptor class A domains.
    Contains 37 LDL-receptor class B repeats.
  • Cellular localization

    Membrane. Membrane > coated pit.
  • Information by UniProt
  • Database links

  • Alternative names

    • Calcium sensor protein antibody
    • DBS antibody
    • Glycoprotein 330 antibody
    • gp330 antibody
    • Heymann nephritis antigen homolog antibody
    • Low-density lipoprotein receptor-related protein 2 antibody
    • LRP-2 antibody
    • Lrp2 antibody
    • LRP2_HUMAN antibody
    • Megalin antibody
    see all

Images

  • IHC image of ab76969 staining in human kidney carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76969, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ab76969 staining Lrp2 / Megalin in monkey kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate pH 6.0. Samples were then incubated with ab76969 at 1µg/ml for 1 hour with BSA in TBS. The secondary used was an undiluted HRP conjugated goat anti-rabbit polyclonal.

    See Abreview

  • ab76969 staining Lrp2 / Megalin in mouse kidney tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, blocked for 60 minutes at 25°C and antigen retrieval was by heat mediation. The sample was incubated with primary antibody (1/1000) at 25°C for 1 hour. An Alexa Fluor® 488-conjugated donkey rabbit polyclonal (1/1200) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Maydan O  et al. Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy. Physiol Rep 6:e13654 (2018). Mouse . Read more (PubMed: 29595914) »
  • Christov M  et al. Inducible podocyte-specific deletion of CTCF drives progressive kidney disease and bone abnormalities. JCI Insight 3:N/A (2018). Read more (PubMed: 29467330) »
See all 12 Publications for this product

Customer reviews and Q&As

1-10 of 14 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney
Blocking step
sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde

Mr. David Ivancic

Verified customer

Submitted Jul 24 2019

Application
Western blot
Sample
Rat Tissue lysate - whole (Rat Retinal Pigment Epithelium)
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Loading amount
40 µg
Specification
Rat Retinal Pigment Epithelium
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Andrew Vargas

Verified customer

Submitted Apr 16 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Retina)
Permeabilization
No
Specification
Retina
Blocking step
Normal Donkey Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Andrew Vargas

Verified customer

Submitted Sep 03 2018

Application
IHC - Wholemount
Sample
Mouse Embryo (Wholemount blastocyst)
Specification
Wholemount blastocyst

Mr. Nicolas Porchet

Verified customer

Submitted Apr 20 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (adult and fetal kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: commercial - ZYTOMED's OmniPrep (pH 9)
Permeabilization
Yes - 0.05% tween 20 in the washing buffer
Specification
adult and fetal kidney
Blocking step
commercial - Invitrogen's CAS-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 03 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: commercial - ZYTOMED's OmniPrep (pH 9)
Permeabilization
No
Specification
mouse kidney
Blocking step
commercial - Invitrogen's CAS-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 03 2015

Application
Western blot
Sample
Rat Tissue lysate - other (Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 19 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain, ependymal cells (ventricle lining cells))
Antigen retrieval step
None
Permeabilization
Yes - 0.05% Tween 20
Specification
Brain, ependymal cells (ventricle lining cells)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Yi-An Yang

Verified customer

Submitted Nov 13 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Kidney, renal tubular cells)
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney, renal tubular cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Yi-An Yang

Verified customer

Submitted Nov 12 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Specification
Kidney
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6.0
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C

Rodolfo Valued Customer

Verified customer

Submitted Apr 16 2013

1-10 of 14 Abreviews or Q&A

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