Anti-Lrp2 / Megalin antibody [CD7D5] (ab184676)
Key features and details
- Mouse monoclonal [CD7D5] to Lrp2 / Megalin
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-Lrp2 / Megalin antibody [CD7D5]
See all Lrp2 / Megalin primary antibodies -
Description
Mouse monoclonal [CD7D5] to Lrp2 / Megalin -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Full length protein corresponding to Human Lrp2/ Megalin.
Database link: P98164 -
Positive control
- Mouse kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
CD7D5 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (4) |
1/100 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/100 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Acts together with cubilin to mediate HDL endocytosis (By similarity). May participate in regulation of parathyroid-hormone and para-thyroid-hormone-related protein release. -
Tissue specificity
Absorptive epithelia, including renal proximal tubules. -
Involvement in disease
Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS) [MIM:222448]; also known as faciooculoacousticorenal syndrome (FOAR syndrome). DBS is a rare autosomal recessive disorder characterized by major malformations including agenesis of the corpus callosum, congenital diaphragmatic hernia, facial dysmorphology, ocular anomalies, sensorineural hearing loss and developmental delay. The FOAR syndrome was first described as comprising facial anomalies, ocular anomalies, sensorineural hearing loss, and proteinuria. DBS and FOAR were first described as distinct disorders but the classic distinguishing features between the 2 disorders were presence of proteinuria and absence of diaphragmatic hernia and corpus callosum anomalies in FOAR. Early reports noted that the 2 disorders shared many phenotypic features and may be identical. Although there is variability in the expression of some features (e.g. agenesis of the corpus callosum and proteinuria), DBS and FOAR are now considered to represent the same entity. -
Sequence similarities
Belongs to the LDLR family.
Contains 17 EGF-like domains.
Contains 36 LDL-receptor class A domains.
Contains 37 LDL-receptor class B repeats. -
Cellular localization
Membrane. Membrane > coated pit. - Information by UniProt
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Database links
- Entrez Gene: 14725 Mouse
- SwissProt: A2ARV4 Mouse
- Unigene: 23847 Mouse
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Alternative names
- Calcium sensor protein antibody
- DBS antibody
- Glycoprotein 330 antibody
see all
Images
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ab184676 staining Lrp2 / Megalin in mouse liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices, permeablized with 0.1 M PBS with 3% Triton X and blocked with 10% serum for 60 minutes at 24°C. The sample was incubated with primary antibody (1/1000 in 0.1M PBST with 10% donkeys serum) at 4°C for 24 hours. An Alexa Fluor® 568-conjugated donkey monoclonal (1/1000) was used as the secondary antibody.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse kidney tissue labeling Lrp2 / Megalin with ab184676 at 1/100 dilution.
Datasheets and documents
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Datasheet download
References (9)
ab184676 has been referenced in 9 publications.
- Wang Y et al. GSDMD-dependent neutrophil extracellular traps promote macrophage-to-myofibroblast transition and renal fibrosis in obstructive nephropathy. Cell Death Dis 13:693 (2022). PubMed: 35941120
- Li Y et al. GSDME-mediated pyroptosis promotes inflammation and fibrosis in obstructive nephropathy. Cell Death Differ 28:2333-2350 (2021). PubMed: 33664482
- Adam RJ et al. Functionally Essential Tubular Proteins Are Lost to Urine-Excreted, Large Extracellular Vesicles during Chronic Renal Insufficiency. Kidney360 1:1105-1115 (2020). PubMed: 34263177
- Watanabe H et al. Expression of Acsm2, a kidney-specific gene, parallels the function and maturation of proximal tubular cells. Am J Physiol Renal Physiol 319:F603-F611 (2020). PubMed: 32830538
- Mukherjee M et al. Notch signaling regulates Akap12 expression and primary cilia length during renal tubule morphogenesis. FASEB J 34:9512-9530 (2020). PubMed: 32474964
- Yin W et al. Protein kinase C and protein kinase A are involved in the protection of recombinant human glucagon-like peptide-1 on glomeruli and tubules in diabetic rats. J Diabetes Investig 10:613-625 (2019). PubMed: 30307132
- Wang P et al. Dissecting the Global Dynamic Molecular Profiles of Human Fetal Kidney Development by Single-Cell RNA Sequencing. Cell Rep 24:3554-3567.e3 (2018). PubMed: 30257215
- Otterpohl KL et al. Nonmuscle myosin 2 proteins encoded byMyh9,Myh10, andMyh14are uniquely distributed in the tubular segments of murine kidney. Physiol Rep 5:N/A (2017). IHC-P ; Mouse . PubMed: 29208685
- Yadav RK et al. TMBIM6 (transmembrane BAX inhibitor motif containing 6) enhances autophagy and reduces renal dysfunction in a cyclosporine A-induced nephrotoxicity model. Autophagy 11:1760-74 (2015). PubMed: 26305401