Anti-Lrp2 / Megalin antibody [CD7D5] (ab184676)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody [CD7D5] (ab184676)](https://www.abcam.com/ps/products/184/ab184676/Images/ab184676-259313-anti-lrp2-megalin-antibody-cd7d5-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody [CD7D5] (ab184676)")
Key features and details
- Mouse monoclonal [CD7D5] to Lrp2 / Megalin
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Lrp2 / Megalin antibody [CD7D5]
See all Lrp2 / Megalin primary antibodies -
Description
Mouse monoclonal [CD7D5] to Lrp2 / Megalin -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Full length protein corresponding to Human Lrp2/ Megalin.
Database link: P98164 -
Positive control
- Mouse kidney tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
CD7D5 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab184676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Acts together with cubilin to mediate HDL endocytosis (By similarity). May participate in regulation of parathyroid-hormone and para-thyroid-hormone-related protein release. -
Tissue specificity
Absorptive epithelia, including renal proximal tubules. -
Involvement in disease
Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS) [MIM:222448]; also known as faciooculoacousticorenal syndrome (FOAR syndrome). DBS is a rare autosomal recessive disorder characterized by major malformations including agenesis of the corpus callosum, congenital diaphragmatic hernia, facial dysmorphology, ocular anomalies, sensorineural hearing loss and developmental delay. The FOAR syndrome was first described as comprising facial anomalies, ocular anomalies, sensorineural hearing loss, and proteinuria. DBS and FOAR were first described as distinct disorders but the classic distinguishing features between the 2 disorders were presence of proteinuria and absence of diaphragmatic hernia and corpus callosum anomalies in FOAR. Early reports noted that the 2 disorders shared many phenotypic features and may be identical. Although there is variability in the expression of some features (e.g. agenesis of the corpus callosum and proteinuria), DBS and FOAR are now considered to represent the same entity. -
Sequence similarities
Belongs to the LDLR family.
Contains 17 EGF-like domains.
Contains 36 LDL-receptor class A domains.
Contains 37 LDL-receptor class B repeats. -
Cellular localization
Membrane. Membrane > coated pit. - Information by UniProt
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Database links
- Entrez Gene: 4036 Human
- Entrez Gene: 14725 Mouse
- Entrez Gene: 29216 Rat
- Omim: 600073 Human
- SwissProt: P98164 Human
- SwissProt: A2ARV4 Mouse
- SwissProt: P98158 Rat
- Unigene: 657729 Human
see all -
Alternative names
- Calcium sensor protein antibody
- DBS antibody
- Glycoprotein 330 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody [CD7D5] (ab184676)This image is courtesy of an Abreview by Ruma Raha-Chowdhury.
ab184676 staining Lrp2 / Megalin in mouse liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices, permeablized with 0.1 M PBS with 3% Triton X and blocked with 10% serum for 60 minutes at 24°C. The sample was incubated with primary antibody (1/1000 in 0.1M PBST with 10% donkeys serum) at 4°C for 24 hours. An Alexa Fluor® 568-conjugated donkey monoclonal (1/1000) was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody [CD7D5] (ab184676)](https://www.abcam.com/ps/products/184/ab184676/Images/ab184676-215634-anti-lrp2-megalin-antibody-cd7d5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody [CD7D5] (ab184676)Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse kidney tissue labeling Lrp2 / Megalin with ab184676 at 1/100 dilution.
Datasheets and documents
References (4)
ab184676 has been referenced in 4 publications.
- Yin W et al. Protein kinase C and protein kinase A are involved in the protection of recombinant human glucagon-like peptide-1 on glomeruli and tubules in diabetic rats. J Diabetes Investig 10:613-625 (2019). PubMed: 30307132
- Wang P et al. Dissecting the Global Dynamic Molecular Profiles of Human Fetal Kidney Development by Single-Cell RNA Sequencing. Cell Rep 24:3554-3567.e3 (2018). PubMed: 30257215
- Otterpohl KL et al. Nonmuscle myosin 2 proteins encoded byMyh9,Myh10, andMyh14are uniquely distributed in the tubular segments of murine kidney. Physiol Rep 5:N/A (2017). IHC-P ; Mouse . PubMed: 29208685
- Yadav RK et al. TMBIM6 (transmembrane BAX inhibitor motif containing 6) enhances autophagy and reduces renal dysfunction in a cyclosporine A-induced nephrotoxicity model. Autophagy 11:1760-74 (2015). PubMed: 26305401
