Key features and details
- Mouse monoclonal [CD7D5] to Lrp2 / Megalin
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-Lrp2 / Megalin antibody [CD7D5]
See all Lrp2 / Megalin primary antibodies
DescriptionMouse monoclonal [CD7D5] to Lrp2 / Megalin
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Full length protein corresponding to Human Lrp2/ Megalin.
Database link: P98164
- Mouse kidney tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityProtein G purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Our Abpromise guarantee covers the use of ab184676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
FunctionActs together with cubilin to mediate HDL endocytosis (By similarity). May participate in regulation of parathyroid-hormone and para-thyroid-hormone-related protein release.
Tissue specificityAbsorptive epithelia, including renal proximal tubules.
Involvement in diseaseDefects in LRP2 are the cause of Donnai-Barrow syndrome (DBS) [MIM:222448]; also known as faciooculoacousticorenal syndrome (FOAR syndrome). DBS is a rare autosomal recessive disorder characterized by major malformations including agenesis of the corpus callosum, congenital diaphragmatic hernia, facial dysmorphology, ocular anomalies, sensorineural hearing loss and developmental delay. The FOAR syndrome was first described as comprising facial anomalies, ocular anomalies, sensorineural hearing loss, and proteinuria. DBS and FOAR were first described as distinct disorders but the classic distinguishing features between the 2 disorders were presence of proteinuria and absence of diaphragmatic hernia and corpus callosum anomalies in FOAR. Early reports noted that the 2 disorders shared many phenotypic features and may be identical. Although there is variability in the expression of some features (e.g. agenesis of the corpus callosum and proteinuria), DBS and FOAR are now considered to represent the same entity.
Sequence similaritiesBelongs to the LDLR family.
Contains 17 EGF-like domains.
Contains 36 LDL-receptor class A domains.
Contains 37 LDL-receptor class B repeats.
Cellular localizationMembrane. Membrane > coated pit.
- Information by UniProt
- Calcium sensor protein antibody
- DBS antibody
- Glycoprotein 330 antibody
ab184676 staining Lrp2 / Megalin in mouse liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices, permeablized with 0.1 M PBS with 3% Triton X and blocked with 10% serum for 60 minutes at 24°C. The sample was incubated with primary antibody (1/1000 in 0.1M PBST with 10% donkeys serum) at 4°C for 24 hours. An Alexa Fluor® 568-conjugated donkey monoclonal (1/1000) was used as the secondary antibody.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse kidney tissue labeling Lrp2 / Megalin with ab184676 at 1/100 dilution.
ab184676 has been referenced in 4 publications.
- Yin W et al. Protein kinase C and protein kinase A are involved in the protection of recombinant human glucagon-like peptide-1 on glomeruli and tubules in diabetic rats. J Diabetes Investig 10:613-625 (2019). PubMed: 30307132
- Wang P et al. Dissecting the Global Dynamic Molecular Profiles of Human Fetal Kidney Development by Single-Cell RNA Sequencing. Cell Rep 24:3554-3567.e3 (2018). PubMed: 30257215
- Otterpohl KL et al. Nonmuscle myosin 2 proteins encoded byMyh9,Myh10, andMyh14are uniquely distributed in the tubular segments of murine kidney. Physiol Rep 5:N/A (2017). IHC-P ; Mouse . PubMed: 29208685
- Yadav RK et al. TMBIM6 (transmembrane BAX inhibitor motif containing 6) enhances autophagy and reduces renal dysfunction in a cyclosporine A-induced nephrotoxicity model. Autophagy 11:1760-74 (2015). PubMed: 26305401