Anti-Lrp2 / Megalin antibody [CD7D5] (ab184676)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-Lrp2 / Megalin antibody [CD7D5]
See all Lrp2 / Megalin primary antibodies -
Description
Mouse monoclonal [CD7D5] to Lrp2 / Megalin -
Host species
Mouse -
Tested applications
Suitable for: IHC-FoFr, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Full length protein corresponding to Human Lrp2/ Megalin.
Database link: P98164 -
Positive control
- Mouse kidney tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
CD7D5 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab184676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FoFr | Use at an assay dependent concentration. | |
| IHC-P | 1/100 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. | |
| IHC-Fr | 1/100 - 1/200. |
Target
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Function
Acts together with cubilin to mediate HDL endocytosis (By similarity). May participate in regulation of parathyroid-hormone and para-thyroid-hormone-related protein release. -
Tissue specificity
Absorptive epithelia, including renal proximal tubules. -
Involvement in disease
Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS) [MIM:222448]; also known as faciooculoacousticorenal syndrome (FOAR syndrome). DBS is a rare autosomal recessive disorder characterized by major malformations including agenesis of the corpus callosum, congenital diaphragmatic hernia, facial dysmorphology, ocular anomalies, sensorineural hearing loss and developmental delay. The FOAR syndrome was first described as comprising facial anomalies, ocular anomalies, sensorineural hearing loss, and proteinuria. DBS and FOAR were first described as distinct disorders but the classic distinguishing features between the 2 disorders were presence of proteinuria and absence of diaphragmatic hernia and corpus callosum anomalies in FOAR. Early reports noted that the 2 disorders shared many phenotypic features and may be identical. Although there is variability in the expression of some features (e.g. agenesis of the corpus callosum and proteinuria), DBS and FOAR are now considered to represent the same entity. -
Sequence similarities
Belongs to the LDLR family.
Contains 17 EGF-like domains.
Contains 36 LDL-receptor class A domains.
Contains 37 LDL-receptor class B repeats. -
Cellular localization
Membrane. Membrane > coated pit. - Information by UniProt
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Database links
- Entrez Gene: 4036 Human
- Entrez Gene: 14725 Mouse
- Entrez Gene: 29216 Rat
- Omim: 600073 Human
- SwissProt: P98164 Human
- SwissProt: A2ARV4 Mouse
- SwissProt: P98158 Rat
- Unigene: 657729 Human
see all -
Alternative names
- Calcium sensor protein antibody
- DBS antibody
- Glycoprotein 330 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody [CD7D5] (ab184676)](https://www.abcam.com/ps/products/184/ab184676/Images/ab184676-259313-anti-lrp2-megalin-antibody-cd7d5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody [CD7D5] (ab184676)This image is courtesy of an Abreview by Ruma Raha-Chowdhury.ab184676 staining Lrp2 / Megalin in mouse liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices, permeablized with 0.1 M PBS with 3% Triton X and blocked with 10% serum for 60 minutes at 24°C. The sample was incubated with primary antibody (1/1000 in 0.1M PBST with 10% donkeys serum) at 4°C for 24 hours. An Alexa Fluor® 568-conjugated donkey monoclonal (1/1000) was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody [CD7D5] (ab184676)](https://www.abcam.com/ps/products/184/ab184676/Images/ab184676-215634-anti-lrp2-megalin-antibody-cd7d5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody [CD7D5] (ab184676)Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse kidney tissue labeling Lrp2 / Megalin with ab184676 at 1/100 dilution.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Yin W et al. Protein kinase C and protein kinase A are involved in the protection of recombinant human glucagon-like peptide-1 on glomeruli and tubules in diabetic rats. J Diabetes Investig 10:613-625 (2019). Read more (PubMed: 30307132) »
- Wang P et al. Dissecting the Global Dynamic Molecular Profiles of Human Fetal Kidney Development by Single-Cell RNA Sequencing. Cell Rep 24:3554-3567.e3 (2018). Read more (PubMed: 30257215) »
