Key features and details
- Goat polyclonal to LRP4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LRP4 antibody
See all LRP4 primary antibodies
DescriptionGoat polyclonal to LRP4
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- Human brain (cerebral cortex) lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab85697 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab85697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 216 kDa).
1 hour primary incubation is recommended for this product.
FunctionPotential cell surface endocytic receptor, which binds and internalizes extracellular ligands for degradation by lysosomes. Involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway.
Tissue specificityExpressed in several regions of the brain.
Involvement in diseaseDefects in LRP4 are the cause of Cenani-Lenz syndactyly syndrome (CLSS) [MIM:212780]. It is a congenital malformation syndrome defined as complete and complex syndactyly of the hands combined with malformations of the forearm bones and similar manifestations in the lower limbs.
Sequence similaritiesBelongs to the LDLR family.
Contains 3 EGF-like domains.
Contains 8 LDL-receptor class A domains.
Contains 20 LDL-receptor class B repeats.
- Information by UniProt
- KIAA0816 antibody
- LDLR dan antibody
- Low density lipoprotein receptor related protein 4 antibody
Anti-LRP4 antibody (ab85697) at 0.1 µg/ml + Human cerebral cortex lysate (in RIPA buffer) at 35 µg
Predicted band size: 216 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
ab85697 has been referenced in 2 publications.
- Selcen D et al. Impaired Synaptic Development, Maintenance, and Neuromuscular Transmission in LRP4-Related Myasthenia. JAMA Neurol 72:889-96 (2015). PubMed: 26052878
- Ohkawara B et al. LRP4 third ß-propeller domain mutations cause novel congenital myasthenia by compromising agrin-mediated MuSK signaling in a position-specific manner. Hum Mol Genet N/A:N/A (2013). PubMed: 24234652