Overview

  • Product name

  • Description

    Goat polyclonal to LRP4
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide:

    C-SNPSYRTSTQEVK

    , corresponding to internal sequence amino acids 1765-1777 of Human LRP4 (NP_002325.2).

  • Positive control

    • Human brain (cerebral cortex) lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab85697 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 216 kDa).

Target

  • Function

    Potential cell surface endocytic receptor, which binds and internalizes extracellular ligands for degradation by lysosomes. Involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway.
  • Tissue specificity

    Expressed in several regions of the brain.
  • Involvement in disease

    Defects in LRP4 are the cause of Cenani-Lenz syndactyly syndrome (CLSS) [MIM:212780]. It is a congenital malformation syndrome defined as complete and complex syndactyly of the hands combined with malformations of the forearm bones and similar manifestations in the lower limbs.
  • Sequence similarities

    Belongs to the LDLR family.
    Contains 3 EGF-like domains.
    Contains 8 LDL-receptor class A domains.
    Contains 20 LDL-receptor class B repeats.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA0816 antibody
    • LDLR dan antibody
    • Low density lipoprotein receptor related protein 4 antibody
    • Low-density lipoprotein receptor-related protein 4 antibody
    • LRP-4 antibody
    • LRP10 antibody
    • Lrp4 antibody
    • LRP4_HUMAN antibody
    • MEGF7 antibody
    • Multiple epidermal growth factor like domains 7 antibody
    • Multiple epidermal growth factor-like domains 7 antibody
    see all

Images

  • Anti-LRP4 antibody (ab85697) at 0.1 µg/ml + Human cerebral cortex lysate (in RIPA buffer) at 35 µg

    Predicted band size: 216 kDa
    Observed band size: 200 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.

References

This product has been referenced in:

  • Selcen D  et al. Impaired Synaptic Development, Maintenance, and Neuromuscular Transmission in LRP4-Related Myasthenia. JAMA Neurol 72:889-96 (2015). Read more (PubMed: 26052878) »
  • Ohkawara B  et al. LRP4 third ß-propeller domain mutations cause novel congenital myasthenia by compromising agrin-mediated MuSK signaling in a position-specific manner. Hum Mol Genet N/A:N/A (2013). Read more (PubMed: 24234652) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab85697.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up