Key features and details
- Mouse monoclonal [N207/27] to LRP4 - N-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG2a
Product nameAnti-LRP4 antibody [N207/27] - N-terminal
See all LRP4 primary antibodies
DescriptionMouse monoclonal [N207/27] to LRP4 - N-terminal
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Fusion protein (His-tag) corresponding to Mouse LRP4 aa 26-350 (N terminal). Extracellular domain.
ACGRSHFTCAVSALGECTCIPAQWQCDGDNDCGDHSDEDGCTLPTCSPLD FHCDNGKCIRRSWVCDGDNDCEDDSDEQDCPPRECEEDEFPCQNGYCIRS LWHCDGDNDCGDNSDEQCDMRKCSDKEFRCSDGSCIAEHWYCDGDTDCKD GSDEESCPSAVPSPPCNLEEFQCAYGRCILDIYHCDGDDDCGDWSDESDC SSHQPCRSGEFMCDSGLCINSGWRCDGDADCDDQSDERNCTTSMCTAEQF RCRSGRCVRLSWRCDGEDDCADNSDEENCENTGSPQCASDQFLCWNGRCI GQRKLCNGINDCGDSSDESPQQNCR
Database link: Q8VI56
- WB: Rat brain membrane lysate. ICC/IF: SK-N-BE cells.
The clone number has been updated from S207-27 to N207/27, both clone numbers name the same antibody clone.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab174637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 212 kDa.|
FunctionPotential cell surface endocytic receptor, which binds and internalizes extracellular ligands for degradation by lysosomes. Involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway.
Tissue specificityExpressed in several regions of the brain.
Involvement in diseaseDefects in LRP4 are the cause of Cenani-Lenz syndactyly syndrome (CLSS) [MIM:212780]. It is a congenital malformation syndrome defined as complete and complex syndactyly of the hands combined with malformations of the forearm bones and similar manifestations in the lower limbs.
Sequence similaritiesBelongs to the LDLR family.
Contains 3 EGF-like domains.
Contains 8 LDL-receptor class A domains.
Contains 20 LDL-receptor class B repeats.
- Information by UniProt
- KIAA0816 antibody
- LDLR dan antibody
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4% paraformaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for LRP4 (green) using ab174637 at 1/100 dilution in ICC/IF. Secondary antibody is Goat Anti-Mouse ATTO 488 at 1:100. Actin is detected with Phalloidin Texas Red F-Actin stain (red). The nuclear counterstain is DAPI (blue).
Lane 1 : Anti-LRP4 antibody [N207/27] - N-terminal (ab174637) at 1/500 dilution
Lane 2 : Anti-LRP4 antibody [N207/27] - N-terminal (ab174637) at 1/1000 dilution
Lane 3 : Anti-LRP4 antibody [N207/27] - N-terminal (ab174637) at 1/2000 dilution
All lanes : Rat brain membrane lysate
Predicted band size: 212 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab174637 has been referenced in 1 publication.
- Smuder AJ et al. Cervical spinal cord injury exacerbates ventilator-induced diaphragm dysfunction. J Appl Physiol (1985) 120:166-77 (2016). PubMed: 26472866