Recombinant Anti-LRP5 antibody [EPR22477-218] (ab223203)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22477-218] to LRP5
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-LRP5 antibody [EPR22477-218]
See all LRP5 primary antibodies -
Description
Rabbit monoclonal [EPR22477-218] to LRP5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF7, HCT 116, MEF, 3T3-L1 and HeLa whole cell lysates; rat liver tissue lysate; His-tagged mouse LRP5 recombinant protein (aa 1407-1614); 293T transfected with LRP5 overexpression vector whole cell lysate. IP: 3T3-L1 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22477-218 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/10000. Detects a band of approximately 180-200 kDa (predicted molecular weight: 179 kDa).
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IP |
1/30.
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Notes |
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WB
1/500 - 1/10000. Detects a band of approximately 180-200 kDa (predicted molecular weight: 179 kDa). |
IP
1/30. |
Target
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Function
Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Appears be required for postnatal control of vascular regression in the eye. Required for posterior patterning of the epiblast during gastrulation. -
Tissue specificity
Widely expressed, with the highest level of expression in the liver. -
Involvement in disease
Defects in LRP5 are the cause of vitreoretinopathy exudative type 4 (EVR4) [MIM:601813]. EVR4 is a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina. This may lead to compensatory retinal neovascularization, which is thought to be induced by hypoxia from the initial avascular insult. New vessels are prone to leakage and rupture causing exudates and bleeding, followed by scarring, retinal detachment and blindness. Clinical features can be highly variable, even within the same family. Patients with mild forms of the disease are asymptomatic, and their only disease related abnormality is an arc of avascular retina in the extreme temporal periphery. EVR4 inheritance can be autosomal dominant or recessive.
Genetic variations in LRP5 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
Defects in LRP5 are the cause of osteoporosis-pseudoglioma syndrome (OPPG) [MIM:259770]; also known as osteogenesis imperfecta ocular form. OPPG is a recessive disorder characterized by very low bone mass and blindness. Individualy with OPPG are prone to develop bone fractures and deformations and have various eye abnormalities, including phthisis bulbi, retinal detachments, falciform folds or persistent vitreal vasculature.
Defects in LRP5 are a cause of high bone mass trait (HBM) [MIM:601884]. HBM is a rare phenotype characterized by exceptionally dense bones. HBM individuals show otherwise a completely normal skeletal structure and no other unusual clinical findings.
Defects in LRP5 are a cause of endosteal hyperostosis Worth type (WENHY) [MIM:144750]; also known as autosomal dominant osteosclerosis. WENHY is an autosomal dominant sclerosing bone dysplasia clinically characterized by elongation of the mandible, increased gonial angle, flattened forehead, and the presence of a slowly enlarging osseous prominence of the hard palate (torus palatinus). Serum calcium, phosphorus and alkaline phosphatase levels are normal. Radiologically, it is characterized by early thickening of the endosteum of long bones, the skull and of the mandible. With advancing age, the trabeculae of the metaphysis become thickened. WENHY becomes clinically and radiologically evident by adolescence, does not cause deformity except in the skull and mandible, and is not associated with bone pain or fracture. Affected patients have normal height, proportion, intelligence and longevity.
Defects in LRP5 are the cause of osteopetrosis autosomal dominant type 1 (OPTA1) [MIM:607634]. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. OPTA1 is characterized by generalized osteosclerosis most pronounced in the cranial vault. Patients are often asymptomatic, but some suffer from pain and hearing loss. It appears to be the only type of osteopetrosis not associated with an increased fracture rate.
Defects in LRP5 are the cause of van Buchem disease type 2 (VBCH2)[MIM:607636]. VBCH2 is an autosomal dominant sclerosing bone dysplasia characterized by cranial osteosclerosis, thickened calvaria and cortices of long bones, enlarged mandible and normal serum alkaline phosphatase levels. -
Sequence similarities
Belongs to the LDLR family.
Contains 4 EGF-like domains.
Contains 3 LDL-receptor class A domains.
Contains 20 LDL-receptor class B repeats. -
Post-translational
modificationsPhosphorylation of cytoplasmic PPPSP motifs regulates the signal transduction of the Wnt signaling pathway through acting as a docking site for AXIN1. -
Cellular localization
Membrane. Endoplasmic reticulum. Chaperoned to the plasma membrane by MESD. - Information by UniProt
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Database links
- Entrez Gene: 4041 Human
- Entrez Gene: 16973 Mouse
- Entrez Gene: 293649 Rat
- Omim: 603506 Human
- SwissProt: O75197 Human
- SwissProt: Q91VN0 Mouse
- Unigene: 6347 Human
- Unigene: 274581 Mouse
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Alternative names
- BMND1 antibody
- EVR1 antibody
- EVR4 antibody
see all
Images
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All lanes : Anti-LRP5 antibody [EPR22477-218] (ab223203) at 1/500 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : LRP5 knockout HEK293T cell lysate
Lane 3 : SW620 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 179 kDa
Observed band size: 180-200 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab223203 observed at 180-200 kDa. Red - loading control ab7291 observed at 50 kDa.
ab223203 Anti-LRP5 antibody [EPR22477-218] was shown to specifically react with LRP5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266618 (knockout cell lysate ab257202) was used. Wild-type and LRP5 knockout samples were subjected to SDS-PAGE. ab223203 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-LRP5 antibody [EPR22477-218] (ab223203) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 4 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 179 kDa
Exposure time: 3 minutesThe expression profile observed is consistent with what has been described in the literature (PMID: 25808845).
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-LRP5 antibody [EPR22477-218] (ab223203) at 1/1000 dilution
Lane 1 : MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate at 20 µg
Lane 2 : Untreated 3T3-L1 (mouse embryonic fibroblast), whole cell lysate at 20 µg
Lane 3 : 3T3-L1 differentiated adipocytes whole cell lysate 20ug
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 179 kDa
Observed band size: 180-200 kDa why is the actual band size different from the predicted?The expression profile observed is consistent with what has been described in the literature (PMID: 25808845).
Differentiation procedure: https://www.abcam.com/protocols/differentiation-of-3t3-l1-cells-into-adipocyte-like-cells-protocol
Exposure times: Lane 1: 3 mins; Lane 2-3: 26 secs.
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-LRP5 antibody [EPR22477-218] (ab223203) at 1/1000 dilution
Lane 1 : His-tagged mouse LRP5 recombinant protein (aa 1407-1614)
Lane 2 : His-tagged mouse LRP6 recombinant protein (aa 1394-1613)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 179 kDa
Observed band size: 25.1,37.5 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: lane 1: 5.5 secs; lane 2: 59secs
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LRP5 was immunoprecipitated from 0.35 mg 3T3-L1 (mouse embryonic fibroblast) differentiated adipocytes whole cell lysate 10µg with ab223203 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab223203. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: 3T3-L1 (mouse embryonic fibroblast) differentiated adipocytes whole cell lysate 10µg.
Lane 2: ab223203 IP in 3T3-L1 differentiated adipocytes whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223203 in 3T3-L1 differentiated adipocytes whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 mins.
Differentiation procedure: https://www.abcam.com/protocols/differentiation-of-3t3-l1-cells-into-adipocyte-like-cells-protocol.
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Lanes 1-3 : Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] (ab76417) at 1/10000 dilution
Lanes 4-6 : Anti-LRP5 antibody [EPR22477-218] (ab223203) at 1/10000 dilution
Lanes 1 & 4 : 293T transfected with blank vector whole cell lysate
Lanes 2 & 5 : 293T transfected with LRP6 overexpression vector whole cell lysate
Lanes 3 & 6 : 293T transfected with LRP5 overexpression vector whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 179 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and dilution buffer: 5%NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab223203 has been referenced in 2 publications.
- Bian Y et al. Astragalus membranaceus (Huangqi) and Rhizoma curcumae (Ezhu) decoction suppresses colorectal cancer via downregulation of Wnt5/β-Catenin signal. Chin Med 17:11 (2022). PubMed: 34991661
- Lin J et al. LRP5-Mediated Lipid Uptake Modulates Osteogenic Differentiation of Bone Marrow Mesenchymal Stromal Cells. Front Cell Dev Biol 9:766815 (2021). PubMed: 34796178