Product nameAnti-LRP5L antibody
See all LRP5L primary antibodies
DescriptionRabbit polyclonal to LRP5L
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- HEK293T transfected lysate. Human skeletal muscle tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122013 in the following tested applications.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|WB||1/250 - 1/500.|
|IHC-P||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesContains 5 LDL-receptor class B repeats.
- Information by UniProt
- Low density lipoprotein receptor-related protein 5 like antibody
- Low-density lipoprotein receptor-related protein 5-like protein antibody
- LRP-5-like antibody
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122013 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-LRP5L antibody (ab122013) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T lysate
Lane 2 : LRP5L (co-expressed with a C-terminal myc-DDK tag ~3.1 kDa) transfected HEK293T lysate
Developed using the ECL technique.
ab122013, at a 1/10 dilution, staining Human LRP5L in skeletal muscle, using Immunohistochemistry, Formalin/PFA-fixed parraffin-embedded tissue. Moderate cytoplasmic positivity in myocytes shown.
ab122013 has not yet been referenced specifically in any publications.