Anti-LRP6 antibody (ab24386)

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Key features and details

  • Goat polyclonal to LRP6
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-LRP6 antibody
    See all LRP6 primary antibodies

  • Description

    Goat polyclonal to LRP6

  • Host species

    Goat

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Dog, Chimpanzee

  • Immunogen

    Synthetic peptide:

    CMTSVATAKGYTSDL

    , corresponding to amino acids 1546-1560 of Human LRP6

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation.

  • Tissue specificity

    Widely co-expressed with LRP5 during embryogenesis and in adult tissues.

  • Involvement in disease

    Defects in LRP6 are the cause of autosomal dominant coronary artery disease type 2 (ADCAD2) [MIM:610947].

  • Sequence similarities

    Belongs to the LDLR family.
    Contains 4 EGF-like domains.
    Contains 3 LDL-receptor class A domains.
    Contains 20 LDL-receptor class B repeats.

  • Domain

    The YWTD-EGF-like domains 1 and 2 are required for the interaction with Wnt-frizzled complex. The YWTD-EGF-like domains 3 and 4 are required for the interaction with DKK1.
    The PPPSP motifs play a central role in signal transduction by being phosphorylated, leading to activate the Wnt signaling pathway.

  • Post-translational
    modifications

    Dual phosphorylation of cytoplasmic PPPSP motifs sequentially by GSK3 and CK1 is required for AXIN1-binding, and subsequent stabilization and activation of beta-catenin via preventing GSK3-mediated phosphorylation of beta-catenin. Phosphorylated, in vitro, by GRK5/6 within and outside the PPPSP motifs. Phosphorylation at Ser-1490 by CDK14 during G2/M phase leads to regulation of the Wnt signaling pathway during the cell cycle. Phosphorylation by GSK3B is induced by RPSO1 binding and inhibited by DKK1. Phosphorylated, in vitro, by casein kinase I on Thr-1479.
    Undergoes gamma-secretase-dependent regulated intramembrane proteolysis (RIP). The extracellular domain is first released by shedding, and then, through the action of gamma-secretase, the intracellular domain (ICD) is released into the cytoplasm where it is free to bind to GSK3B and to activate canonical Wnt signaling.
    Palmitoylation on the two sites near the transmembrane domain leads to release of LRP6 from the endoplasmic reticulum.
    Mono-ubiquitinated which retains LRP6 in the endoplasmic reticulum.
    N-glycosylation is required for cell surface location.

  • Cellular localization

    Membrane. Endoplasmic reticulum. On Wnt signaling, undergoes a cycle of caveolin- or clathrin-mediated endocytosis and plasma membrane location. Released from the endoplasmic reticulum on palmitoylation. Mono-ubiquitination retains it in the endoplasmic reticulum in the absence of palmitoylation. On Wnt signaling, phosphorylated, aggregates and colocalizes with AXIN1 and GSK3B at the plasma membrane in LRP6-signalsomes. Chaperoned to the plasma membrane by MESD.
  • Information by UniProt

  • Database links

    • Alternative names

      • ADCAD2 antibody
      • C030016K15Rik antibody
      • Cd antibody
      • FLJ90062 antibody
      • FLJ90421 antibody
      • LDL receptor related protein 6 antibody
      • Low density lipoprotein receptor related protein 6 antibody
      • Low-density lipoprotein receptor-related protein 6 antibody
      • LRP-6 antibody
      • LRP6 antibody
      • LRP6_HUMAN antibody
      • OTTHUMP00000238979 antibody
      • OTTHUMP00000238980 antibody
      • OTTHUMP00000238982 antibody
      • STHAG7 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRP6 antibody (ab24386)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRP6 antibody (ab24386)
      Ab24386 (5ug/ml) staining human LRP6 in human liver tissue by immunohistochemistry using paraffin embedded tissue.

      Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

      In paraffin embedded Human Liver shows strong cytoplasmic staining in hepatocytes and cytoplasmic staining in bile ductulus cells.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRP6 antibody (ab24386)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRP6 antibody (ab24386)
      ab24386 staining LRP6 in Human Kidney at 4µg/ml by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Sections were subjected to steamed antigen retrieval with citrate buffer pH 9.0, and antibody was visualised with HRP-staining.

    References

    Publishing research using ab24386? Please let us know so that we can cite the reference in this datasheet.

    ab24386 has been referenced in 5 publications.

    • Wang XM  et al. Role and mechanisms of action of microRNA-21 as regards the regulation of the WNT/ß-catenin signaling pathway in the pathogenesis of non-alcoholic fatty liver disease. Int J Mol Med 44:2201-2212 (2019). PubMed: 31638173
    • Li CP  et al. Mutation of miR-21 targets endogenous lipoprotein receptor-related protein 6 and nonalcoholic fatty liver disease. Am J Transl Res 9:715-721 (2017). PubMed: 28337300
    • Du C  et al. MiR-126-3p suppresses tumor metastasis and angiogenesis of hepatocellular carcinoma by targeting LRP6 and PIK3R2. J Transl Med 12:259 (2014). PubMed: 25240815
    • Tahir SA  et al. Caveolin-1-LRP6 signaling module stimulates aerobic glycolysis in prostate cancer. Cancer Res 73:1900-11 (2013). Human . PubMed: 23302227
    • Tawk M  et al. Wnt/beta-catenin signaling is an essential and direct driver of myelin gene expression and myelinogenesis. J Neurosci 31:3729-42 (2011). PubMed: 21389228

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question


    I appreciate your email however I was seeking a more direct answer regarding the two specific antibodies that I referenced. Other researchers in the lab I am working in have had bad experiences with the "Abpromise" and the vague description of what is guaranteed. Before we purchase antibodies we want a more explicit guarantee that Abcam will stand behind their products.

    Please give me a clear answer if ab24386 and ab75732 are guaranteed for paraffin embedded mouse tissue.

    Read More
    Answer

    Thanks for your reply.

    I am sorry to hear some of your colleagues have had a bad experience regarding our "Abpromise". I would encourage them to contact us to resolve any issues they have.

    The Abpromise landing page to which I sent you a link does have quite a bit of text on it and its easy to miss the key points regarding your question. Below I have pasted these relevant items from the Abpromise page:

    Replacement or refund for products not performing as stated on the datasheet
    "Predicted to react" information on datasheets is based on sequence homology and provided for reference only
    "Predicted to react" species are not guaranteed, as they have not been tested



    To expand on the above, we guarantee the antibodies to work in the applications listed in the 'Tested application' section and to react with the species listed in the 'Cross reactivity' section.

    How does the Abpromise apply to ab24386?:
    We guarantee it to work in IHC-P and to react with Human LRP6 protein containing the epitope CMTSVATAKGYTSDL, corresponding to amino acids 1546-1560 of Human LRP6.

    Protein orthologs that contain a region which has at least 95% identity to the above epitope, but have not yet been tested for reactivity, are listed in the 'Predicted to work with' section. For ab24386, these species include Mouse, Rat, Chicken, Dog, Chimpanzee. As noted above, "Predicted to react" species are not guaranteed, as they have not been tested.

    How does the Abpromise apply to ab75732?:
    We guarantee it to work in ICC/IF, IHC-P, Flow Cyt, WB, ELISA andICCand to react with Human and MouseLRP6 protein containing an epitope found within amino acids 700-730 of the human protein.

    What does the guarantee mean for you:
    If, after doing your experiment, the antibody does not appear to work as expected (ie. as noted above), we ask you to contact us within 6 months of receiving the antibody. At this point we will offer troubleshooting tipsand may ask further questions to help determine if it is the antibody or perhaps another aspect of the experiment that is the issue. If in the end it does appear that the antibody is the problem, we are happy to offer a replacement with any primary antibody, credit for a future purchase or refund.

    I hope this is helpful. Please contact me if you have any further questions.

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    Question


    We are interested in using a couple of your antibodies for a research project involving paraffin embedded mouse tissues and IHC, and would like to be clear on what you guarantee and what happens if the antibody does not perform as promised. The two antibodies we want a guarantee for are ab75732 and ab24386. Again we are using IHC on paraffin embedded mouse tissues for both and possibly IF for ab75732 as it is advertised. If you could get back to me on you guarantee and refund policy for both of those antibodies it would help us greatly.

    Read More
    Answer

    Thank you for your enquiry.

    Please see the link below for complete details regarding our guarantee, or Abpromise as we call it:
    https://www.abcam.com/abpromise

    I hope this is helpful. Please contact me again if you have any further questions.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com