Anti-LRP6 antibody (ab24386)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-LRP6 antibody
See all LRP6 primary antibodies -
DescriptionGoat polyclonal to LRP6
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Host speciesGoat
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Tested applicationsSuitable for: IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Dog, Chimpanzee -
Immunogen
Synthetic peptide:
CMTSVATAKGYTSDL
, corresponding to amino acids 1546-1560 of Human LRP6
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3 -
Concentration information loading...
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PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab24386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | Use at an assay dependent concentration. |
Target
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FunctionComponent of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation.
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Tissue specificityWidely co-expressed with LRP5 during embryogenesis and in adult tissues.
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Involvement in diseaseDefects in LRP6 are the cause of autosomal dominant coronary artery disease type 2 (ADCAD2) [MIM:610947].
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Sequence similaritiesBelongs to the LDLR family.
Contains 4 EGF-like domains.
Contains 3 LDL-receptor class A domains.
Contains 20 LDL-receptor class B repeats. -
DomainThe YWTD-EGF-like domains 1 and 2 are required for the interaction with Wnt-frizzled complex. The YWTD-EGF-like domains 3 and 4 are required for the interaction with DKK1.
The PPPSP motifs play a central role in signal transduction by being phosphorylated, leading to activate the Wnt signaling pathway. -
Post-translational
modificationsDual phosphorylation of cytoplasmic PPPSP motifs sequentially by GSK3 and CK1 is required for AXIN1-binding, and subsequent stabilization and activation of beta-catenin via preventing GSK3-mediated phosphorylation of beta-catenin. Phosphorylated, in vitro, by GRK5/6 within and outside the PPPSP motifs. Phosphorylation at Ser-1490 by CDK14 during G2/M phase leads to regulation of the Wnt signaling pathway during the cell cycle. Phosphorylation by GSK3B is induced by RPSO1 binding and inhibited by DKK1. Phosphorylated, in vitro, by casein kinase I on Thr-1479.
Undergoes gamma-secretase-dependent regulated intramembrane proteolysis (RIP). The extracellular domain is first released by shedding, and then, through the action of gamma-secretase, the intracellular domain (ICD) is released into the cytoplasm where it is free to bind to GSK3B and to activate canonical Wnt signaling.
Palmitoylation on the two sites near the transmembrane domain leads to release of LRP6 from the endoplasmic reticulum.
Mono-ubiquitinated which retains LRP6 in the endoplasmic reticulum.
N-glycosylation is required for cell surface location. -
Cellular localizationMembrane. Endoplasmic reticulum. On Wnt signaling, undergoes a cycle of caveolin- or clathrin-mediated endocytosis and plasma membrane location. Released from the endoplasmic reticulum on palmitoylation. Mono-ubiquitination retains it in the endoplasmic reticulum in the absence of palmitoylation. On Wnt signaling, phosphorylated, aggregates and colocalizes with AXIN1 and GSK3B at the plasma membrane in LRP6-signalsomes. Chaperoned to the plasma membrane by MESD.
- Information by UniProt
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Database links
- Entrez Gene: 4040 Human
- Entrez Gene: 16974 Mouse
- Omim: 603507 Human
- SwissProt: O75581 Human
- SwissProt: O88572 Mouse
- Unigene: 584775 Human
- Unigene: 658913 Human
- Unigene: 321990 Mouse
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Alternative names
- ADCAD2 antibody
- C030016K15Rik antibody
- Cd antibody
see all
Images
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Ab24386 (5ug/ml) staining human LRP6 in human liver tissue by immunohistochemistry using paraffin embedded tissue.
Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
In paraffin embedded Human Liver shows strong cytoplasmic staining in hepatocytes and cytoplasmic staining in bile ductulus cells. -
ab24386 staining LRP6 in Human Kidney at 4µg/ml by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Sections were subjected to steamed antigen retrieval with citrate buffer pH 9.0, and antibody was visualised with HRP-staining.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Li CP et al. Mutation of miR-21 targets endogenous lipoprotein receptor-related protein 6 and nonalcoholic fatty liver disease. Am J Transl Res 9:715-721 (2017). Read more (PubMed: 28337300) »
- Du C et al. MiR-126-3p suppresses tumor metastasis and angiogenesis of hepatocellular carcinoma by targeting LRP6 and PIK3R2. J Transl Med 12:259 (2014). Read more (PubMed: 25240815) »