Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Loading amount
40 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-Tween 0.1%
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Invitrogen, Donkey anti-Rabbit IgG (H+L) Highly Cr
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 790
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 790
Detection
Detection method
Fluorescence (Odissey)
Exposure
7 minute(s) and 0 second(s)
Bands
Specific: kDa Non-specific: 100 kDa
Additional data
Additional Notes
Predicted molecular weight: 179 kDa
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Jan 23 2020