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Signal Transduction Adapters Transmembrane
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Anti-LRP6 (phospho S1490) antibody (ab226758)

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Western blot - Anti-LRP6 (phospho S1490) antibody (ab226758)

    Key features and details

    • Rabbit polyclonal to LRP6 (phospho S1490)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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    Overview

    • Product name

      Anti-LRP6 (phospho S1490) antibody
      See all LRP6 primary antibodies
    • Description

      Rabbit polyclonal to LRP6 (phospho S1490)
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Xenopus laevis, Rhesus monkey
    • Immunogen

      Synthetic peptide within Human LRP6 (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
      Database link: O75581

    • Positive control

      • WB: HeLa whole cell extract.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.025% Proclin 300
      Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Adapters
      • Transmembrane
      • Stem Cells
      • Signaling Pathways
      • Wnt
      • Surface Molecules

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HeLa whole cell lysate (ab150035)
      • HeLa whole cell lysate (ab29545)

    Applications

    Our Abpromise guarantee covers the use of ab226758 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/3000. Predicted molecular weight: 180 kDa.

    Target

    • Function

      Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation.
    • Tissue specificity

      Widely co-expressed with LRP5 during embryogenesis and in adult tissues.
    • Involvement in disease

      Defects in LRP6 are the cause of autosomal dominant coronary artery disease type 2 (ADCAD2) [MIM:610947].
    • Sequence similarities

      Belongs to the LDLR family.
      Contains 4 EGF-like domains.
      Contains 3 LDL-receptor class A domains.
      Contains 20 LDL-receptor class B repeats.
    • Domain

      The YWTD-EGF-like domains 1 and 2 are required for the interaction with Wnt-frizzled complex. The YWTD-EGF-like domains 3 and 4 are required for the interaction with DKK1.
      The PPPSP motifs play a central role in signal transduction by being phosphorylated, leading to activate the Wnt signaling pathway.
    • Post-translational
      modifications

      Dual phosphorylation of cytoplasmic PPPSP motifs sequentially by GSK3 and CK1 is required for AXIN1-binding, and subsequent stabilization and activation of beta-catenin via preventing GSK3-mediated phosphorylation of beta-catenin. Phosphorylated, in vitro, by GRK5/6 within and outside the PPPSP motifs. Phosphorylation at Ser-1490 by CDK14 during G2/M phase leads to regulation of the Wnt signaling pathway during the cell cycle. Phosphorylation by GSK3B is induced by RPSO1 binding and inhibited by DKK1. Phosphorylated, in vitro, by casein kinase I on Thr-1479.
      Undergoes gamma-secretase-dependent regulated intramembrane proteolysis (RIP). The extracellular domain is first released by shedding, and then, through the action of gamma-secretase, the intracellular domain (ICD) is released into the cytoplasm where it is free to bind to GSK3B and to activate canonical Wnt signaling.
      Palmitoylation on the two sites near the transmembrane domain leads to release of LRP6 from the endoplasmic reticulum.
      Mono-ubiquitinated which retains LRP6 in the endoplasmic reticulum.
      N-glycosylation is required for cell surface location.
    • Cellular localization

      Membrane. Endoplasmic reticulum. On Wnt signaling, undergoes a cycle of caveolin- or clathrin-mediated endocytosis and plasma membrane location. Released from the endoplasmic reticulum on palmitoylation. Mono-ubiquitination retains it in the endoplasmic reticulum in the absence of palmitoylation. On Wnt signaling, phosphorylated, aggregates and colocalizes with AXIN1 and GSK3B at the plasma membrane in LRP6-signalsomes. Chaperoned to the plasma membrane by MESD.
    • Target information above from: UniProt accession O75581 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4040 Human
      • Entrez Gene: 16974 Mouse
      • Entrez Gene: 695843 Rhesus monkey
      • Omim: 603507 Human
      • SwissProt: O75581 Human
      • SwissProt: O88572 Mouse
      • Unigene: 584775 Human
      • Unigene: 658913 Human
      • Unigene: 321990 Mouse
      see all
    • Alternative names

      • ADCAD2 antibody
      • C030016K15Rik antibody
      • Cd antibody
      • FLJ90062 antibody
      • FLJ90421 antibody
      • LDL receptor related protein 6 antibody
      • Low density lipoprotein receptor related protein 6 antibody
      • Low-density lipoprotein receptor-related protein 6 antibody
      • LRP-6 antibody
      • LRP6 antibody
      • LRP6_HUMAN antibody
      • OTTHUMP00000238979 antibody
      • OTTHUMP00000238980 antibody
      • OTTHUMP00000238982 antibody
      • STHAG7 antibody
      see all

    Images

    • Western blot - Anti-LRP6 (phospho S1490) antibody (ab226758)
      Western blot - Anti-LRP6 (phospho S1490) antibody (ab226758)
      All lanes : Anti-LRP6 (phospho S1490) antibody (ab226758) at 1/1000 dilution

      Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract treated with Calf-intestinal alkaline phosphatase

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 180 kDa



      5% SDS-PAGE gel.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab226758? Please let us know so that we can cite the reference in this datasheet.

    ab226758 has not yet been referenced specifically in any publications.

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