Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody (ab226758)
Key features and details
- Rabbit polyclonal to LRP6 (phospho S1490) + LRP5 (phospho S1503)
- Suitable for: WB, Dot blot, Indirect ELISA
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody -
Description
Rabbit polyclonal to LRP6 (phospho S1490) + LRP5 (phospho S1503) -
Host species
Rabbit -
Tested applications
Suitable for: WB, Dot blot, Indirect ELISAmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Xenopus laevis, Rhesus monkey -
Immunogen
Synthetic peptide within Human LRP6 (C terminal) (phospho S1490). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (Carrier-protein conjugated).
Database link: O75581 -
Positive control
- WB: HeLa whole cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 180 kDa.
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Dot blot |
Use at an assay dependent concentration.
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Indirect ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 180 kDa. |
Dot blot
Use at an assay dependent concentration. |
Indirect ELISA
Use at an assay dependent concentration. |
Target
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Cellular localization
LRP6: Membrane. Endoplasmic reticulum. On Wnt signaling, undergoes a cycle of caveolin- or clathrin-mediated endocytosis and plasma membrane location. Released from the endoplasmic reticulum on palmitoylation. Mono-ubiquitination retains it in the endoplasmic reticulum in the absence of palmitoylation. On Wnt signaling, phosphorylated, aggregates and colocalizes with AXIN1 and GSK3B at the plasma membrane in LRP6-signalsomes. Chaperoned to the plasma membrane by MESD. LRP5: Membrane. Endoplasmic reticulum. Chaperoned to the plasma membrane by MESD. -
Database links
- Entrez Gene: 4040 Human
- Entrez Gene: 4041 Human
- Entrez Gene: 16973 Mouse
- Entrez Gene: 16974 Mouse
- Entrez Gene: 695843 Rhesus monkey
- Omim: 603506 Human
- Omim: 603507 Human
- SwissProt: O75197 Human
see all
Images
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Indirect ELISA showing ab226758 binding to LRP6 (phospho S1490) + LRP5 (phospho S1503) peptides. Plates were coated with Human LRP6 non-phospho peptide, Human LRP6 (phospho S1490) peptide, Human LRP5 non-phospho peptide and Human LRP5 (phospho S1503) peptide at 1000 ng/ml.
Secondary antibody was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) used at 1/2500 dilution. -
Dot blot analysis of LRP6 (phospho S1490) + LRP5 (phospho S1503) using ab226758 at 1:1000 dilution followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.
Lane 1: LRP6 non-phospho peptide
Lane 2: LRP6 (S1490) phospho peptide
Lane 3: LRP5 non-phospho peptide
Lane 4: LRP5 (S1503) phospho peptide
Exposure time: 3 minutes
Blocking and diluting buffer and concentration: 5% NFDM/TBST -
Lanes 1-3 : Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody (ab226758) at 1/1000 dilution
Lanes 4-6 : Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) at 1/5000 dilution
Lanes 1 & 4 : 293T transfected with blank vector whole cell lysate
Lanes 2 & 5 : 293T transfected with LRP6 overexpression vector whole cell lysate
Lanes 3 & 6 : 293T transfected with LRP5 overexpression vector whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 180 kDa
Observed band size: 180 kDa
Exposure time: 5 secondsBlocking and diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody (ab226758) at 1/1000 dilution
Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract treated with Calf-intestinal alkaline phosphatase
Lysates/proteins at 30 µg per lane.
Predicted band size: 180 kDa5% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226758 has not yet been referenced specifically in any publications.