Recombinant Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] - BSA and Azide free (ab247469)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2360Y] to LRP6 (phospho S1490) + LRP5 (phospho S1503) - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] - BSA and Azide free -
Description
Rabbit monoclonal [EP2360Y] to LRP6 (phospho S1490) + LRP5 (phospho S1503) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa cell lysates treated with Calyculin, 293T transfected with LRP6 and LRP5 overexpression vector whole cell lysates.
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General notes
ab247469 is the carrier-free version of ab76417.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2360Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 179 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 179 kDa. |
Target
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Cellular localization
LRP6: Membrane. Endoplasmic reticulum. On Wnt signaling, undergoes a cycle of caveolin- or clathrin-mediated endocytosis and plasma membrane location. Released from the endoplasmic reticulum on palmitoylation. Mono-ubiquitination retains it in the endoplasmic reticulum in the absence of palmitoylation. On Wnt signaling, phosphorylated, aggregates and colocalizes with AXIN1 and GSK3B at the plasma membrane in LRP6-signalsomes. Chaperoned to the plasma membrane by MESD. LRP5: Membrane. Endoplasmic reticulum. Chaperoned to the plasma membrane by MESD. -
Database links
- Entrez Gene: 4040 Human
- Entrez Gene: 4041 Human
- Omim: 603506 Human
- Omim: 603507 Human
- SwissProt: O75197 Human
- SwissProt: O75581 Human
- Unigene: 584775 Human
- Unigene: 6347 Human
see all
Images
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Lanes 1-3 : Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] (ab76417) at 1/10000 dilution
Lanes 4-6 : Anti-LRP5 antibody [EPR22477-218] (ab223203) at 1/10000 dilution
Lanes 1 & 4 : 293T transfected with blank vector whole cell lysate
Lanes 2 & 5 : 293T transfected with LRP6 overexpression vector whole cell lysate
Lanes 3 & 6 : 293T transfected with LRP5 overexpression vector whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 179 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and dilution buffer: 5%NFDM/TBST.
This data was developed using ab76417, the same antibody clone in a different buffer formulation.
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All lanes : Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] (ab76417) at 1/1000 dilution
Lane 1 : HeLa cell lysates, untreated
Lane 2 : HeLa cell lysates treated with Calyculin
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 179 kDa
Observed band size: 210 kDa why is the actual band size different from the predicted?This data was developed using ab76417, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab247469 has not yet been referenced specifically in any publications.