Key features and details
- Rabbit polyclonal to LRPAP1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LRPAP1 antibody
See all LRPAP1 primary antibodies
DescriptionRabbit polyclonal to LRPAP1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide corresponding to Human LRPAP1 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Recombinant Human LRPAP1 protein (ab123156) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: HEK293 Whole Cell; A498 Whole Cell; Human Brain Tissue; U-87 MG Whole Cell.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).|
FunctionInteracts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330.
Involvement in diseaseNote=In complex with the alpha-2-MR or gp330, it may have some role in the pathogenesis of membrane glomerular nephritis.
Sequence similaritiesBelongs to the alpha-2-MRAP family.
Cellular localizationEndoplasmic reticulum. Cytoplasm. Cell surface. Intracellular and associated with cell surface receptors. Found in the endoplasmic reticulum.
- Information by UniProt
- 39 kDa receptor-associated protein antibody
- A2MRAP antibody
- A2RAP antibody
All lanes : Anti-LRPAP1 antibody (ab85591) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 3 : Human brain tissue lysate - total protein (ab29466)
Lane 4 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Additional bands at: 37 kDa, 50 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
ICC/IF image of ab85591 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85591, µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (10 min) HeLa cells at 5µg/ml.
IHC image of LRPAP1 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85591, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ab85591 has not yet been referenced specifically in any publications.