Recombinant
RabMAb

Recombinant Anti-LRPAP1 antibody [EPR3328] (ab133488)

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Overview

  • Product name

    Anti-LRPAP1 antibody [EPR3328]
    See all LRPAP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3328] to LRPAP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human LRPAP1 (UniProt ID: P30533).

  • Positive control

    • Human fetal kidney, 293T, A549, BxPC3 and NCCIT lysates; Human brain and kidney tissues

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab133488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.

    • Target

    • Function

      Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330.

    • Involvement in disease

      Note=In complex with the alpha-2-MR or gp330, it may have some role in the pathogenesis of membrane glomerular nephritis.

    • Sequence similarities

      Belongs to the alpha-2-MRAP family.

    • Post-translational
      modifications

      N-glycosylated.

    • Cellular localization

      Endoplasmic reticulum. Cytoplasm. Cell surface. Intracellular and associated with cell surface receptors. Found in the endoplasmic reticulum.
    • Information by UniProt

    • Database links

      • Alternative names

        • 39 kDa receptor-associated protein antibody
        • A2MRAP antibody
        • A2RAP antibody
        • Alpha 2 macroglobulin receptor associated protein antibody
        • Alpha 2 MRAP antibody
        • Alpha-2-macroglobulin receptor-associated protein antibody
        • Alpha-2-MRAP antibody
        • AMRP_HUMAN antibody
        • HBP44 antibody
        • Lipoprotein receptor associated protein antibody
        • Low density lipoprotein receptor related protein associated protein 1 antibody
        • Low density lipoprotein receptor-related protein-associated protein 1 antibody
        • Low density lipoprotein related protein associated protein 1 alpha 2 macroglobulin receptor associated protein antibody
        • Low density lipoprotein related protein associated protein 1 antibody
        • low density lipoprotein-related protein-associated protein 1 (alpha-2-macroglobulin receptor-associated protein 1) antibody
        • Lrpap1 antibody
        • MGC138272 antibody
        • MRAP antibody
        • MYP23 antibody
        • RAP antibody
        see all

      Images

      • Western blot - Anti-LRPAP1 antibody [EPR3328] (ab133488)
        Western blot - Anti-LRPAP1 antibody [EPR3328] (ab133488)
        All lanes : Anti-LRPAP1 antibody [EPR3328] (ab133488) at 1/1000 dilution

        Lane 1 : Human fetal kidney lysate
        Lane 2 : 293T lysate
        Lane 3 : A549 lysate
        Lane 4 : BxPC3 lysate
        Lane 5 : NCCIT lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 41 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3328] (ab133488)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3328] (ab133488)

        Immunohistochemical analysis of LRPAP1 in paraffin embedded Human kidney tissue, using ab133488 at a 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3328] (ab133488)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3328] (ab133488)

        Immunohistochemical analysis of LRPAP1 in paraffin embedded Human brain tissue, using ab133488 at a 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      References

      ab133488 has not yet been referenced specifically in any publications.

      Publishing research using ab133488? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Question

      will send his sequence
      align with human
      check which Ab might be best choice

      human, WB

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      Answer

      Thank you for contacting us.

      As we discussed over the phone, please send me your sequence so that I can find an antibody that works best for your purposes.

      I look forward to hear back and help you further.

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