Recombinant Anti-LRPAP1 antibody [EPR3328] (ab133488)
![Western blot - Anti-LRPAP1 antibody [EPR3328] (ab133488)](https://www.abcam.com/ps/products/133/ab133488/Images/ab133488-147143-anti-lrpap1-antibody-epr3328-western-blot.jpg "Western blot - Anti-LRPAP1 antibody [EPR3328] (ab133488)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3328] to LRPAP1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-LRPAP1 antibody [EPR3328]
See all LRPAP1 primary antibodies -
Description
Rabbit monoclonal [EPR3328] to LRPAP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to residues in Human LRPAP1 (UniProt ID: P30533).
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Positive control
- Human fetal kidney, 293T, A549, BxPC3 and NCCIT lysates; Human brain and kidney tissues
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3328 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330. -
Involvement in disease
Note=In complex with the alpha-2-MR or gp330, it may have some role in the pathogenesis of membrane glomerular nephritis. -
Sequence similarities
Belongs to the alpha-2-MRAP family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Endoplasmic reticulum. Cytoplasm. Cell surface. Intracellular and associated with cell surface receptors. Found in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 4043 Human
- Omim: 104225 Human
- SwissProt: P30533 Human
- Unigene: 40966 Human
- Unigene: 533136 Human
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Alternative names
- 39 kDa receptor-associated protein antibody
- A2MRAP antibody
- A2RAP antibody
see all
Images
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Western blot - Anti-LRPAP1 antibody [EPR3328] (ab133488)All lanes : Anti-LRPAP1 antibody [EPR3328] (ab133488) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : 293T lysate
Lane 3 : A549 lysate
Lane 4 : BxPC3 lysate
Lane 5 : NCCIT lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 41 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3328] (ab133488)](https://www.abcam.com/ps/products/133/ab133488/Images/ab133488-147142-anti-lrpap1-antibody-epr3328-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3328] (ab133488)Immunohistochemical analysis of LRPAP1 in paraffin embedded Human kidney tissue, using ab133488 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3328] (ab133488)](https://www.abcam.com/ps/products/133/ab133488/Images/ab133488-147141-anti-lrpap1-antibody-epr3328-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3328] (ab133488)Immunohistochemical analysis of LRPAP1 in paraffin embedded Human brain tissue, using ab133488 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-LRPAP1 antibody [EPR3328] (ab133488)](https://www.abcam.com/ps/products/133/ab133488/Images/ab133488-4-benefits-of-recombinant-antibodies.png)
Anti-LRPAP1 antibody [EPR3328] (ab133488)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab133488 has not yet been referenced specifically in any publications.