Key features and details
- Rabbit polyclonal to LRPPRC/GP130
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LRPPRC/GP130 antibody
See all LRPPRC/GP130 primary antibodies
DescriptionRabbit polyclonal to LRPPRC/GP130
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
This product was previously labelled as LRPPRC
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab205022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 158 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay play a role in RNA metabolism in both nuclei and mitochondria. In the nucleus binds to HNRPA1-associated poly(A) mRNAs and is part of nmRNP complexes at late stages of mRNA maturation which are possibly associated with nuclear mRNA export. May bind mature mRNA in the nucleus outer membrane. In mitochondria binds to poly(A) mRNA. Plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. May be involved in transcription regulation. Cooperates with PPARGC1A to regulate certain mitochondrially encoded genes and gluconeogenic genes and may regulate docking of PPARGC1A to transcription factors. Seems to be involved in the transcription regulation of the multidrug-related genes MDR1 and MVP. Part of a nuclear factor that binds to the invMED1 element of MDR1 and MVP gene promoters. Binds single-stranded DNA.
Tissue specificityExpressed ubiquitously. Expression is highest in heart, skeletal muscle, kidney and liver, intermediate in brain, non-mucosal colon, spleen and placenta, and lowest in small intestine, thymus, lung and peripheral blood leukocytes.
Involvement in diseaseLeigh syndrome French-Canadian type
Sequence similaritiesContains 20 PPR (pentatricopeptide) repeats.
Cellular localizationMitochondrion. Nucleus, nucleoplasm. Nucleus inner membrane. Nucleus outer membrane. Seems to be predominantly mitochondrial.
- Information by UniProt
- 130 kDa leucine-rich protein antibody
- gp130 antibody
- Leucine-rich PPR motif-containing protein antibody
Immunoprecipitates of 1 mg of 293T lysates labeling LRPPRC after precipitation with positive control (lane 1), ab205022 (lane 2) or control IgG (lane 3) used at 6 µg per reaction. Western blotting was performed with ab205022 at 0.4 µg/mL.
All lanes : Anti-LRPPRC/GP130 antibody (ab205022) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 158 kDa
Exposure time: 3 minutes
ab205022 has been referenced in 1 publication.
- Xu X et al. lncRNA ZEB1-AS1 Mediates Oxidative Low-Density Lipoprotein-Mediated Endothelial Cells Injury by Post-transcriptional Stabilization of NOD2. Front Pharmacol 10:397 (2019). PubMed: 31040785