Overview

  • Product name

    Anti-LRPPRC/GP130 antibody
    See all LRPPRC/GP130 primary antibodies
  • Description

    Rabbit polyclonal to LRPPRC/GP130
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide within Human LRPPRC/GP130 aa 1344-1394. The exact sequence is proprietary.
    Sequence:

    LTAKNTK LDDLFLKRYA SLLKYAGEPV PFIEPPESFE FYAQQLRKLR ENSS


    Database link: NP_573566.2

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysate (ab7899).
  • General notes

     This product was previously labelled as LRPPRC

     

Properties

Applications

Our Abpromise guarantee covers the use of ab205022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 158 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    May play a role in RNA metabolism in both nuclei and mitochondria. In the nucleus binds to HNRPA1-associated poly(A) mRNAs and is part of nmRNP complexes at late stages of mRNA maturation which are possibly associated with nuclear mRNA export. May bind mature mRNA in the nucleus outer membrane. In mitochondria binds to poly(A) mRNA. Plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. May be involved in transcription regulation. Cooperates with PPARGC1A to regulate certain mitochondrially encoded genes and gluconeogenic genes and may regulate docking of PPARGC1A to transcription factors. Seems to be involved in the transcription regulation of the multidrug-related genes MDR1 and MVP. Part of a nuclear factor that binds to the invMED1 element of MDR1 and MVP gene promoters. Binds single-stranded DNA.
  • Tissue specificity

    Expressed ubiquitously. Expression is highest in heart, skeletal muscle, kidney and liver, intermediate in brain, non-mucosal colon, spleen and placenta, and lowest in small intestine, thymus, lung and peripheral blood leukocytes.
  • Involvement in disease

    Leigh syndrome French-Canadian type
  • Sequence similarities

    Contains 20 PPR (pentatricopeptide) repeats.
  • Cellular localization

    Mitochondrion. Nucleus, nucleoplasm. Nucleus inner membrane. Nucleus outer membrane. Seems to be predominantly mitochondrial.
  • Information by UniProt
  • Database links

  • Alternative names

    • 130 kDa leucine-rich protein antibody
    • gp130 antibody
    • Leucine-rich PPR motif-containing protein antibody
    • Leucine-rich PPR-motif containing protein antibody
    • LPPRC_HUMAN antibody
    • LRP 130 antibody
    • lrpprc antibody
    • mitochondrial antibody
    see all

Images

  • Immunoprecipitates of 1 mg of 293T lysates labeling LRPPRC  after precipitation with positive control (lane 1), ab205022 (lane 2) or control IgG (lane 3) used at 6 µg per reaction.  Western blotting was performed with ab205022 at 0.4 µg/mL.

  • All lanes : Anti-LRPPRC/GP130 antibody (ab205022) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 158 kDa


    Exposure time: 3 minutes

References

This product has been referenced in:

  • Xu X  et al. lncRNA ZEB1-AS1 Mediates Oxidative Low-Density Lipoprotein-Mediated Endothelial Cells Injury by Post-transcriptional Stabilization of NOD2. Front Pharmacol 10:397 (2019). Read more (PubMed: 31040785) »
See 1 Publication for this product

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