Anti-LRR-1 antibody (ab169947)

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Overview

  • Product name
    Anti-LRR-1 antibody
    See all LRR-1 primary antibodies
  • Description
    Rabbit polyclonal to LRR-1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human LRR-1 aa 95-242.
    Sequence:

    AISSSLKGFLSAMRLAHRGCNVDTPVSTLTPVKTSEFENFKTKMVITSKK DYPLSKNFPYSLEHLQTSYCGLVRVDMRMLCLKSLRKLDLSHNHIKKLPA TIGDLIHLQELNLNDNHLESFSVALCHSTLQKSLRSLDLSKNKIKALP


    Database link: Q96L50

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Recombinant Human LRR-1 protein (ab165190) can be used as a positive control in WB. Human fetal brain lysate, NIH-3T3 cell lysate, Human pancreas cancer tissue.
  • General notes

    Previously labelled as PPIL5.

Properties

  • Form
    Lyophilised:Reconstitute in 200ul sterile water
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 98% PBS, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/100 - 1/500.

Target

  • Function
    May negatively regulate the 4-1BB-mediated signaling cascades which result in the activation of NK-kappaB and JNK1. Probable substrate recognition subunit of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • Tissue specificity
    Ubiquitous. Maximal expression was seen in the heart and skeletal muscle and minimal expression seen in the kidney.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 7 LRR (leucine-rich) repeats.
  • Information by UniProt
  • Database links
    • Alternative names
      • 4 1BB mediated signaling molecule antibody
      • 4 1BBlrr antibody
      • 4-1BB-mediated-signaling molecule antibody
      • 4-1BBlrr antibody
      • Cyclophilin like 5 antibody
      • Leucine-rich repeat protein 1 antibody
      • LLR1_HUMAN antibody
      • LRR 1 antibody
      • LRR repeat protein 1 antibody
      • LRR-1 antibody
      • LRR-repeat protein 1 antibody
      • LRR1 antibody
      • MGC20689 antibody
      • peptidylprolyl isomerase (cyclophilin) like 5 antibody
      • Peptidylprolyl isomerase like 5 antibody
      • Peptidylprolyl isomerase-like 5 antibody
      • PPIL5 antibody
      see all

    Images

    • Western blot - Anti-LRR-1 antibody (ab169947)
      Western blot - Anti-LRR-1 antibody (ab169947)
      All lanes : Anti-LRR-1 antibody (ab169947) at 1/500 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : NIH-3T3 cell lysate

      Predicted band size: 47 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRR-1 antibody (ab169947)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRR-1 antibody (ab169947)

      Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded Human pancreas cancer tissue labeling LRR-1 with ab169947 at 1/100 dilution

    References

    ab169947 has not yet been referenced specifically in any publications.

    Publishing research using ab169947? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-LRR-1 antibody

     Poor
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (MCF10A cells)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    MCF10A cells
    Blocking step
    10% FBS, 1% BSA, 0.1% Triton X-100, 0.01% NaN3 in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

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    Verified customer

    Submitted Jan 22 2019

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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