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    lrr-1-antibody-ab169947.pdf

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Signal Transduction Growth Factors/Hormones TNF
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Anti-LRR-1 antibody (ab169947)

  • Datasheet
  • SDS
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Western blot - Anti-LRR-1 antibody (ab169947)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRR-1 antibody (ab169947)

Key features and details

  • Rabbit polyclonal to LRR-1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-LRR-1 antibody
    See all LRR-1 primary antibodies
  • Description

    Rabbit polyclonal to LRR-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human LRR-1 aa 95-242.
    Sequence:

    AISSSLKGFLSAMRLAHRGCNVDTPVSTLTPVKTSEFENFKTKMVITSKK DYPLSKNFPYSLEHLQTSYCGLVRVDMRMLCLKSLRKLDLSHNHIKKLPA TIGDLIHLQELNLNDNHLESFSVALCHSTLQKSLRSLDLSKNKIKALP


    Database link: Q96L50
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Human fetal brain lysate and NIH-3T3 cell lysate; IHC: Human pancreas cancer tissue.
  • General notes

    Previously labelled as PPIL5.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Lyophilized:Reconstitute in 200ul sterile water
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 98% PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • TNF

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab169947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/100 - 1/500.

Target

  • Function

    May negatively regulate the 4-1BB-mediated signaling cascades which result in the activation of NK-kappaB and JNK1. Probable substrate recognition subunit of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • Tissue specificity

    Ubiquitous. Maximal expression was seen in the heart and skeletal muscle and minimal expression seen in the kidney.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 7 LRR (leucine-rich) repeats.
  • Target information above from: UniProt accession Q96L50 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 122769 Human
    • Omim: 609193 Human
    • SwissProt: Q96L50 Human
    • Unigene: 451090 Human
    • Alternative names

      • 4 1BB mediated signaling molecule antibody
      • 4 1BBlrr antibody
      • 4-1BB-mediated-signaling molecule antibody
      • 4-1BBlrr antibody
      • Cyclophilin like 5 antibody
      • Leucine-rich repeat protein 1 antibody
      • LLR1_HUMAN antibody
      • LRR 1 antibody
      • LRR repeat protein 1 antibody
      • LRR-1 antibody
      • LRR-repeat protein 1 antibody
      • LRR1 antibody
      • MGC20689 antibody
      • peptidylprolyl isomerase (cyclophilin) like 5 antibody
      • Peptidylprolyl isomerase like 5 antibody
      • Peptidylprolyl isomerase-like 5 antibody
      • PPIL5 antibody
      see all

    Images

    • Western blot - Anti-LRR-1 antibody (ab169947)
      Western blot - Anti-LRR-1 antibody (ab169947)
      All lanes : Anti-LRR-1 antibody (ab169947) at 1/500 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : NIH-3T3 cell lysate

      Predicted band size: 47 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRR-1 antibody (ab169947)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRR-1 antibody (ab169947)

      Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded Human pancreas cancer tissue labeling LRR-1 with ab169947 at 1/100 dilution

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab169947? Please let us know so that we can cite the reference in this datasheet.

    ab169947 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Immunocytochemistry/ Immunofluorescence abreview for Anti-LRR-1 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (MCF10A cells)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    MCF10A cells
    Blocking step
    10% FBS, 1% BSA, 0.1% Triton X-100, 0.01% NaN3 in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 22 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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