Key features and details
- Rabbit polyclonal to LRR-1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LRR-1 antibody
See all LRR-1 primary antibodies
DescriptionRabbit polyclonal to LRR-1
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
- ICC/IF: PC-3 cells. WB: Human tonsil lysate.
Previously labelled as PPIL5.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab221772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 47 kDa.|
FunctionMay negatively regulate the 4-1BB-mediated signaling cascades which result in the activation of NK-kappaB and JNK1. Probable substrate recognition subunit of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Tissue specificityUbiquitous. Maximal expression was seen in the heart and skeletal muscle and minimal expression seen in the kidney.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 7 LRR (leucine-rich) repeats.
- Information by UniProt
- 4 1BB mediated signaling molecule antibody
- 4 1BBlrr antibody
- 4-1BB-mediated-signaling molecule antibody
PFA-fixed, Triton X-100 permeabilized PC-3 (human prostate adenocarcinoma cell line) cells stained for LRR-1 (green) using ab221772 at 4 µg/ml in ICC/IF.
Anti-LRR-1 antibody (ab221772) at 0.4 µg/ml (Human testis tissue lysate)
Predicted band size: 47 kDa
Western blot analysis of human testis tissue lysate with ab221772 at 0.4 µg/ml.
ab221772 has not yet been referenced specifically in any publications.