Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8188(2)] to LRRC15
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Product nameAnti-LRRC15 antibody [EPR8188(2)]
See all LRRC15 primary antibodies
DescriptionRabbit monoclonal [EPR8188(2)] to LRRC15
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human LRRC15 aa 50-150. The exact sequence is proprietary.
- SH-SY5Y and fetal brain lysates; SH-SY5Y cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab150376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 64 kDa.|
|ICC/IF||1/50 - 1/100.|
Tissue specificityBrain and placenta.
Sequence similaritiesContains 15 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
- Information by UniProt
- hLib antibody
- Leucine-rich repeat protein induced by beta-amyloid homolog antibody
- Leucine-rich repeat-containing protein 15 antibody
All lanes : Anti-LRRC15 antibody [EPR8188(2)] (ab150376) at 1/1000 dilution
Lanes 1 & 4 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
Lane 2 : Human brain lysates
Lane 3 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
Immunocytochemistry/ Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell) cells labeling LRRC15 with purified ab150376 at 1:100 dilution (9.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunofluorescent analysis of SH-SY5Y cells labeling LRRC15 with ab150376 (unpurified) at a 1/50 dilution.
All lanes : Anti-LRRC15 antibody [EPR8188(2)] (ab150376) at 1/1000 dilution (unpurified)
Lane 1 : SH-SY5Y cell lysate
Lane 2 : fetal brain cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labeled goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 64 kDa
ab150376 has been referenced in 1 publication.
- Giovani PA et al. Membrane proteome characterization of periodontal ligament cell sets from deciduous and permanent teeth. J Periodontol 90:775-787 (2019). PubMed: 30499115