Overview

  • Product name

  • Description

    Rabbit polyclonal to LRRC25/MAPA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human LRRC25/MAPA aa 36-85 (N terminal).
    Sequence:

    AEFSATCLNFSGLSLSLPHNQSLRASNVILLDLSGNGLRELPVTFFAHLQ

  • Positive control

    • Human fetal brain lysate.
  • General notes

     This product was previously labelled as LRRC25

     

Properties

Applications

Our Abpromise guarantee covers the use of ab84954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance

    LRRC25 is a single-pass type I membrane protein. It contains 2 LRR (leucine-rich) repeats. LRRC25 may be involved in the activation of cells of innate and acquired immunity.
  • Cellular localization

    Membrane; Single-pass type I membrane protein.
  • Database links

  • Alternative names

    • FLJ38116 antibody
    • Leucine rich repeat containing 25 antibody
    • Leucine rich repeat containing protein 25 antibody
    • LRRC 25 antibody
    • MAPA antibody
    • Monocyte and plasmacytoid activated molecule antibody
    see all

Images

  • Anti-LRRC25/MAPA antibody (ab84954) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 33 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?



    Gel concentration: 12%

References

This product has been referenced in:

  • Feng Y  et al. LRRC25 Functions as an Inhibitor of NF-?B Signaling Pathway by Promoting p65/RelA for Autophagic Degradation. Sci Rep 7:13448 (2017). Read more (PubMed: 29044191) »
See 1 Publication for this product

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