Key features and details
- Mouse monoclonal [OTI1D9] to LRRC25/MAPA
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-LRRC25/MAPA antibody [OTI1D9]
DescriptionMouse monoclonal [OTI1D9] to LRRC25/MAPA
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human LRRC25/MAPA aa 1-305. Produced in HEK-293T cell (NP_660299).
MGGTLAWTLLLPLLLRESDSLEPSCTVSSADVDWNAEFSATCLNFSGLSL SLPHNQSLRASNVILLDLSGNGLRELPVTFFAHLQKLEVLNVLRNPLSRV DGALAARCDLDLQADCNCALESWHDIRRDNCSGQKPLLCWDTTSSQHNLS AFLEVSCAPGLASATIGAVVVSGCLLLGLAIAGPVLAWRLWRCRVARSRE LNKPWAAQDGPKPGLGLQPRYGSRSAPKPQVAVPSCPSTPDYENMFVGQP AAEHQWDEQGAHPSEDNDFYINYKDIDLASQPVYCNLQSLGQAPMDEEEY VIPGH
Database link: Q8N386
- WB: pCMV6-ENTRY LRRC25/MAPA transfected HEK-293T cells. IHC-P: Human lung carcinoma, endometrium adenocarcinoma and lymphoma tissue. ICC/IF: pCMV6-ENTRY LRRC25/MAPA transfected COS-7 cells Flow: HEK-293T cells transfected with overexpressing plasmid.
Clone OTI1D9 (formerly 1D9).
This product was previously labelled as LRRC25
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 33 kDa.|
|IHC-P||1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceLRRC25 is a single-pass type I membrane protein. It contains 2 LRR (leucine-rich) repeats. LRRC25 may be involved in the activation of cells of innate and acquired immunity.
Cellular localizationMembrane; Single-pass type I membrane protein.
- FLJ38116 antibody
- Leucine rich repeat containing 25 antibody
- Leucine rich repeat containing protein 25 antibody
All lanes : Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY LRRC25/MAPA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 33 kDa
Paraffin-embedded human lung carcinoma tissue stained for LRRC25/MAPA using ab236395 at 1/150 in immunohistochemical analysis.
COS-7 (African green monkey kidney fibroblast-like cell line) cells transfected with pCMV6-ENTRY LRRC25/MAPA labeling LRRC25/MAPA using ab236395 at 1/100 dilution in ICC/IF.
Flow cytometric analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue).
Paraffin-embedded human lymphoma tissue stained for LRRC25/MAPA using ab236395 at 1/150 in immunohistochemical analysis.
Paraffin-embedded human endometrium adenocarcinoma tissue stained for LRRC25/MAPA using ab236395 at 1/150 in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236395 has not yet been referenced specifically in any publications.