Product nameAnti-LRRC47 antibody
See all LRRC47 primary antibodies
DescriptionRabbit polyclonal to LRRC47
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
antigen, corresponding to amino acids 348-469 of Human LRRC47 (Q8N1G4).
- Human colon tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesContains 7 LRR (leucine-rich) repeats.
- Information by UniProt
- 2900010D03Rik antibody
- AA960559 antibody
- Leucine-rich repeat-containing protein 47 antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli and cytoplasm. Recommended concentration of ab121283 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemical staining of formalin fixed and paraffin embedded Human colon tissue labelled with ab121283 at 1/100 dilution.
All lanes : Anti-LRRC47 antibody (ab121283) at 1/250 dilution
Lane 1 : RT-4
Lane 2 : U-251 MG
Lane 3 : Human plasma
Lane 4 : Liver
Lane 5 : Tonsil
ab121283 has been referenced in 1 publication.
- Piao JM et al. MicroRNA-381 Favors Repair of Nerve Injury Through Regulation of the SDF-1/CXCR4 Signaling Pathway via LRRC4 in Acute Cerebral Ischemia after Cerebral Lymphatic Blockage. Cell Physiol Biochem 46:890-906 (2018). PubMed: 29669322