Anti-LRRC4C antibody (ab111572)

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Immunocytochemistry/ Immunofluorescence - Anti-LRRC4C antibody (ab111572)
  • Western blot - Anti-LRRC4C antibody (ab111572)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC4C antibody (ab111572)

Key features and details

  • Rabbit polyclonal to LRRC4C
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-LRRC4C antibody
    See all LRRC4C primary antibodies

  • Description

    Rabbit polyclonal to LRRC4C

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human LRRC4C aa 5-195.

  • Positive control

    • NT2D1, IMR32, U-87 MG and MCF7 cells and whole cell lysates; Human breast cancer tissue.

  • General notes

     This product was previously labelled as Netrin G1 ligand

     

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab111572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Zebrafish
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 72 kDa.
ICC/IF
1/100 - 1/500.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternative antigen retrieval method: heat mediated Tris-EDTA buffer, pH 8.0
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 72 kDa.
ICC/IF
1/100 - 1/500.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternative antigen retrieval method: heat mediated Tris-EDTA buffer, pH 8.0

Target

  • Function

    May promote neurite outgrowth of developing thalamic neurons.

  • Tissue specificity

    Highly expressed in the cerebral cortex, including frontal, parietal and occipital lobes. Putamen, amygdala, hippocampus and medulla oblongata show moderate expression. Caudate nucleus and thalamus express small amounts, whereas other brain regions show very weak or no expression.

  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.

  • Domain

    The LRR region is both necessary and sufficient for the interaction with NTNG1.

  • Cellular localization

    Cell junction, synapse, postsynaptic cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 6430556C10Rik antibody
    • KIAA1580 antibody
    • Leucine rich repeat containing 4C antibody
    • Leucine-rich repeat-containing protein 4C antibody
    • LRC4C_HUMAN antibody
    • LRRC4C antibody
    • mKIAA1580 antibody
    • Netrin-G1 ligand antibody
    • NGL 1 antibody
    • NGL-1 antibody
    • NGL1 antibody
    • RGD1311013 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-LRRC4C antibody (ab111572)
    Immunocytochemistry/ Immunofluorescence - Anti-LRRC4C antibody (ab111572)

    ab111572 at 1/500 dilution staining LRRC4C in methanol-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.

  • Western blot - Anti-LRRC4C antibody (ab111572)
    Western blot - Anti-LRRC4C antibody (ab111572)
    Anti-LRRC4C antibody (ab111572) at 1/2000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size: 72 kDa



    7.5% SDS-PAGE.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC4C antibody (ab111572)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC4C antibody (ab111572)

    Paraffin-embedded human breast cancer tissue stained for LRRC4C using ab111572 at 1/250 dilution in Immunohistochemical analysis.

References (0)

Publishing research using ab111572? Please let us know so that we can cite the reference in this datasheet.

ab111572 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-LRRC4C antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Cell (intestinal tissue, swiss roll 14um thickness)
Permeabilization
Yes - 0.1% Triton X-100
Specification
intestinal tissue, swiss roll 14um thickness
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted May 13 2019

Flow Cytometry abreview for Anti-Netrin G1 ligand antibody

 Inconclusive
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (MCF-7)
Permeabilization
No
Specification
MCF-7
Preparation
Cell harvesting/tissue preparation method: harvest using trypsin
Sample buffer: no
Read More

Miss. Hadas Pahima

Verified customer

Submitted Jul 17 2017

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