Anti-LRRC4C antibody (ab111572)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-LRRC4C antibody
See all LRRC4C primary antibodies -
Description
Rabbit polyclonal to LRRC4C -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Zebrafish
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Immunogen
Recombinant fragment corresponding to Human LRRC4C aa 5-195.
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Positive control
- NT2D1, IMR32, U-87 MG and MCF7 cells and whole cell lysates; Human breast cancer tissue.
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General notes
This product was previously labelled as Netrin G1 ligand
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab111572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 72 kDa. | |
| ICC/IF | 1/100 - 1/500. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternative antigen retrieval method: heat mediated Tris-EDTA buffer, pH 8.0 |
Target
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Function
May promote neurite outgrowth of developing thalamic neurons. -
Tissue specificity
Highly expressed in the cerebral cortex, including frontal, parietal and occipital lobes. Putamen, amygdala, hippocampus and medulla oblongata show moderate expression. Caudate nucleus and thalamus express small amounts, whereas other brain regions show very weak or no expression. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 9 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
Domain
The LRR region is both necessary and sufficient for the interaction with NTNG1. -
Cellular localization
Cell junction, synapse, postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 57689 Human
- Entrez Gene: 241568 Mouse
- Entrez Gene: 311236 Rat
- Entrez Gene: 553785 Zebrafish
- Omim: 608817 Human
- SwissProt: Q9HCJ2 Human
- SwissProt: Q8C031 Mouse
- Unigene: 745123 Human
see all -
Alternative names
- 6430556C10Rik antibody
- KIAA1580 antibody
- Leucine rich repeat containing 4C antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-LRRC4C antibody (ab111572)ab111572 at 1/500 dilution staining LRRC4C in methanol-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
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Western blot - Anti-LRRC4C antibody (ab111572)Anti-LRRC4C antibody (ab111572) at 1/2000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 72 kDa
7.5% SDS-PAGE. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC4C antibody (ab111572)Paraffin-embedded human breast cancer tissue stained for LRRC4C using ab111572 at 1/250 dilution in Immunohistochemical analysis.
Protocols
References
ab111572 has not yet been referenced specifically in any publications.
