Anti-LRRC59 antibody (ab127912)
Key features and details
- Rabbit polyclonal to LRRC59
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-LRRC59 antibody
See all LRRC59 primary antibodies -
Description
Rabbit polyclonal to LRRC59 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 3-196 of Human LRRC59 (Q96AG4).
-
Positive control
- A431, HeLa and HepG2 cell lysates; Human U87 xenograft tissue; HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab127912 is purified by antigen-affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab127912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 35 kDa.
|
|
IHC-P |
1/100 - 1/1000.
|
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 35 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
-
Sequence similarities
Contains 5 LRR (leucine-rich) repeats. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
-
Database links
- Entrez Gene: 55379 Human
- Omim: 614854 Human
- SwissProt: Q96AG4 Human
- Unigene: 370927 Human
-
Alternative names
- Leucine rich repeat containing 59 antibody
- Leucine-rich repeat-containing protein 59 antibody
- LRC59_HUMAN antibody
see all
Images
-
All lanes : Anti-LRRC59 antibody (ab127912) at 1/1000 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : LRRC59 Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 35 kDa -
ab127912 at 1/500 dilution staining LRRC58 in paraffin-embedded Human U87 xenograft tissue by Immunohistochemistry.
-
All lanes : Anti-LRRC59 antibody (ab127912) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 35 kDa
10% SDS PAGE -
ab127912 at 1/500 dilution staining LRRC59 in methanol-fixed HeLa cells by confocal Immunofluorescence. Alpha-tubulin filaments are stained in red.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab127912 has been referenced in 2 publications.
- Xian H et al. LRRC59 modulates type I interferon signaling by restraining the SQSTM1/p62-mediated autophagic degradation of pattern recognition receptor DDX58/RIG-I. Autophagy 16:408-418 (2020). PubMed: 31068071
- Li D et al. Overexpression of LRRC59 Is Associated with Poor Prognosis and Promotes Cell Proliferation and Invasion in Lung Adenocarcinoma. Onco Targets Ther 13:6453-6463 (2020). PubMed: 32753886