Overview

  • Product name

  • Description

    Rabbit polyclonal to LRRC59
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 3-196 of Human LRRC59 (Q96AG4).

  • Positive control

    • A431, HeLa and HepG2 cell lysates; Human U87 xenograft tissue; HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab127912 is purified by antigen-affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab127912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Sequence similarities

    Contains 5 LRR (leucine-rich) repeats.
  • Cellular localization

    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Leucine rich repeat containing 59 antibody
    • Leucine-rich repeat-containing protein 59 antibody
    • LRC59_HUMAN antibody
    • LRRC59 antibody
    • p34 antibody
    • PRO1855 antibody
    • Ribosome binding protein p34 antibody
    see all

Images

  • All lanes : Anti-LRRC59 antibody (ab127912) at 1/1000 dilution

    Lane 1 : WT 293T cell extracts
    Lane 2 : LRRC59 Knockout 293T cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 35 kDa

  • ab127912 at 1/500 dilution staining LRRC58 in paraffin-embedded Human U87 xenograft tissue by Immunohistochemistry.
  • All lanes : Anti-LRRC59 antibody (ab127912) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 35 kDa



    10% SDS PAGE
  • ab127912 at 1/500 dilution staining LRRC59 in methanol-fixed HeLa cells by confocal Immunofluorescence. Alpha-tubulin filaments are stained in red.

References

ab127912 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab127912.
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