Key features and details
- Rabbit polyclonal to LRRC8A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LRRC8A antibody
DescriptionRabbit polyclonal to LRRC8A
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig
- Human fetal liver, Jurkat and A549 cell lysates; Human fetal kidney tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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FormLyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab157489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 94 kDa.|
|IHC-P||1/100 - 1/500.|
FunctionInvolved in B-cell development. Required for the pro-B cell to pre-B cell transition.
Tissue specificityExpressed in brain, kidney, ovary, lung, liver, heart, and fetal brain and liver. Found at high levels in bone marrow; lower levels are detected in peripheral blood cells. Expressed on T-cells as well as on B-lineage cells.
Involvement in diseaseDefects in LRRC8A are the cause of agammaglobulinemia type 5 (AGM5) [MIM:613506]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B-cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Note=A chromosomal aberration involving LRRC8 has been found in a patient with congenital agammaglobulinemia. Translocation t(9;20)(q33.2;q12). The translocation truncates the LRRC8 gene, resulting in deletion of the eighth, ninth, and half of the seventh LRR domains.
Sequence similaritiesContains 17 LRR (leucine-rich) repeats.
- Information by UniProt
- AGM5 antibody
- FLJ10337 antibody
- FLJ41617 antibody
All lanes : Anti-LRRC8A antibody (ab157489) at 1/500 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A549 cell lysate
Predicted band size: 94 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fetal kidney tissue labeling LRRC8A with ab157489 at 1/100 dilution.
ab157489 has been referenced in 3 publications.
- Yang C et al. LRRC8A potentiates temozolomide sensitivity in glioma cells via activating mitochondria-dependent apoptotic pathway. Hum Cell 32:41-50 (2019). PubMed: 30426452
- Konishi T et al. LRRC8A Expression Influences Growth of Esophageal Squamous Cell Carcinoma. Am J Pathol 189:1973-1985 (2019). PubMed: 31323188
- Zhang H et al. High expression of leucine-rich repeat-containing 8A is indicative of a worse outcome of colon cancer patients by enhancing cancer cell growth and metastasis. Oncol Rep 40:1275-1286 (2018). PubMed: 30015914