Overview

  • Product name

    Anti-LRRC8C antibody
  • Description

    Rabbit polyclonal to LRRC8C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 662-792 of Human LRRC8C (Q8TDW0).

  • Positive control

    • Human colon tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab122046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May play a role in adipogenesis.
  • Tissue specificity

    Expressed at highest levels in skeletal muscle, and at moderate levels in heart, lung and peripheral blood leukocytes.
  • Sequence similarities

    Contains 17 LRR (leucine-rich) repeats.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AD158 antibody
    • AI326115 antibody
    • E430036I04Rik antibody
    • Factor for adipocyte differentiation 158 antibody
    • FAD158 antibody
    • Leucine-rich repeat-containing protein 8C antibody
    • LRC8C_HUMAN antibody
    • LRRC8C antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in golgi apparatus. Recommended concentration of ab122046 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122046, at 1/20, staining LRRC8C in paraffin embedded Human colon tissue by Immunohistochemistry.

References

ab122046 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Mouse Cell (Mouse T cells)
Permeabilization
Yes - Permeabilization buffer-Biolegend
Gating Strategy
Cells were sorted for mCherry and gated as: CD4+mCherry+
Specification
Mouse T cells
Preparation
Cell harvesting/tissue preparation method: Expanded T cells with CD3+CD28 and transducer to over-express LRRC8C
Sample buffer: Cell were fixed and permeabilized to stain for LRRD C-terminus of LRRC8C
Fixation
Formaldehyde

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Verified customer

Submitted Jul 30 2018

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