Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176587 was affinity purified using an epitope specific to LRRFIP1 immobilized on solid support.
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab176587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 89 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionTranscriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the LRRFIP family.
Developmental stageWidely expressed in fetal tissues.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- FLAP 1 antibody
- FLAP1 antibody
- FLIIAP 1 antibody
Detection of LRRFIP1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176587 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab176587 was used at 0.4 µg/ml. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 1 second.
All lanes : Anti-LRRFIP1 antibody (ab176587) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 89 kDa
Exposure time: 30 seconds
ab176587 has not yet been referenced specifically in any publications.