Overview

  • Product name

  • Description

    Rabbit polyclonal to LRRFIP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human LRRFIP1 (internal sequence). The exact sequence is proprietary.
    Database link: Q32MZ4

  • Positive control

    • WB: HEK-239T, A431 and NCI-H929 whole cell extracts. ICC/IF: A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab229466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 89 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the LRRFIP family.
  • Developmental stage

    Widely expressed in fetal tissues.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLAP 1 antibody
    • FLAP1 antibody
    • FLIIAP 1 antibody
    • FLIIAP1 antibody
    • GC binding factor 2 antibody
    • GC-binding factor 2 antibody
    • GCF 2 antibody
    • GCF2 antibody
    • HUFI 1 antibody
    • HUFI1 antibody
    • Leucine rich repeat (in FLII) interacting protein 1 antibody
    • Leucine rich repeat flightless interacting protein 1 antibody
    • Leucine-rich repeat flightless-interacting protein 1 antibody
    • LRR FLII interacting protein 1 antibody
    • LRR FLII-interacting protein 1 antibody
    • LRRF1_HUMAN antibody
    • LRRFIP 1 antibody
    • Lrrfip1 antibody
    • MGC10947 antibody
    • MGC119738 antibody
    • MGC119739 antibody
    • NEDD8 conjugating enzyme antibody
    • TAR RNA interacting protein antibody
    • TAR RNA-interacting protein antibody
    • Transcription factor 9 like antibody
    • TRIP antibody
    see all

Images

  • A431 (human epidermoid carcinoma cell line) cells stained for LRRFIP1 (green) using ab229466 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Anti-LRRFIP1 antibody (ab229466) at 1/1000 dilution + NCI-H929 (human bone marrow lymphoblast cell line) whole cell extract at 30 µg

    Predicted band size: 89 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-LRRFIP1 antibody (ab229466) at 1/5000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 89 kDa



    7.5% SDS-PAGE gel.

References

ab229466 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229466.
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