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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-LRRFIP2 antibody [377C3a] (ab84874)

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Western blot - Anti-LRRFIP2 antibody [377C3a] (ab84874)

    Key features and details

    • Mouse monoclonal [377C3a] to LRRFIP2
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG2a

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    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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    Overview

    • Product name

      Anti-LRRFIP2 antibody [377C3a]
      See all LRRFIP2 primary antibodies
    • Description

      Mouse monoclonal [377C3a] to LRRFIP2
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Recombinant fragment
    • Immunogen

      Recombinant fragment corresponding to a region within human LRRFIP2

    • Positive control

      • Immunising recombinant protein
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.40
      Preservative: 0.05% Sodium azide
      Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      Purified from from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane.
    • Clonality

      Monoclonal
    • Clone number

      377C3a
    • Isotype

      IgG2a
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Signal Transduction
      • Signaling Pathway
      • Nuclear Signaling
      • NFkB Pathway
      • Stem Cells
      • Signaling Pathways
      • Wnt
      • Cytoplasmic
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • NFkB pathway
      • Immunology
      • Innate Immunity
      • TLR Signaling

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab84874 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 82 kDa.
    Notes
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 82 kDa.

    Target

    • Function

      May function as activator of the canonical Wnt signaling pathway, in association with DVL3, upstream of CTNNB1/beta-catenin.
    • Tissue specificity

      Ubiquitous with higher expression in heart and skeletal muscle.
    • Sequence similarities

      Belongs to the LRRFIP family.
    • Target information above from: UniProt accession Q9Y608 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • 5133400F20Rik antibody
      • AI850587 antibody
      • DKFZp434H2035 antibody
      • FLJ20248 antibody
      • FLJ22683 antibody
      • FLJ58304 antibody
      • HUFI 2 antibody
      • Leucine rich repeat (in FLII) interacting protein 2 antibody
      • Leucine rich repeat flightless interacting protein 2 antibody
      • Leucine-rich repeat flightless-interacting protein 2 antibody
      • LRR FLII interacting protein 2 antibody
      • LRR FLII-interacting protein 2 antibody
      • LRRF2_HUMAN antibody
      • Lrrfip2 antibody
      see all

    Images

    • Western blot - Anti-LRRFIP2 antibody [377C3a] (ab84874)
      Western blot - Anti-LRRFIP2 antibody [377C3a] (ab84874)
      Anti-LRRFIP2 antibody [377C3a] (ab84874) + immunising recombinant protein

      Predicted band size: 82 kDa
      Observed band size: 38 kDa why is the actual band size different from the predicted?



      Note: actual band size differs from predicted one, because the WB is depicting the recombinant fragment used as immunogen.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab84874? Please let us know so that we can cite the reference in this datasheet.

    ab84874 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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