Product nameLRRK2 Antibody Sampler Panel
Species reactivityReacts with: Human
LRRK2 Antibody Sampler Panel ab263464 contains multiple trial-sized versions of anti-human antibody clones against LRRK2, specifically selected for high performance in various applications. All but one of these clones (ab203181) are also reactive against mouse LRRK2. This panel contains 5 recombinant rabbit monoclonal antibodies against LRRK2. They are provided as a sampler panel to allow you to easily evaluate each antibody.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
- Rabbit monoclonal [UDD2 10(12)] to LRRK2 (phospho S935) (20 µL) ab133450
- Rabbit monoclonal [MJFF2 (c41-2)] to LRRK2 (20 µL) ab133474
- Rabbit monoclonal [MJFR-19-7-8] to LRRK2 (phospho S1292) (20 µL) ab203181
- Rabbit monoclonal [UDD3 30(12)] to LRRK2 (20 µL) ab133518
- Rabbit monoclonal [MJFF3 (c69-6)] to LRRK2 (20 µL) ab133475
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Directly conjugated versions of our antibodies are available and ready to use for multicolor flow cytometry or immunocytochemistry analysis. Please refer to the ‘Associated products’ section below.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Please refer to the ‘Associated products’ section below.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 kit ab203181 - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] 2 x 10µl ab133450 - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] 2 x 10µl ab133474 - Anti-LRRK2 antibody [MJFF2 (c41-2)] 2 x 10µl ab133475 - Anti-LRRK2 antibody [MJFF3 (c69-6)] 2 x 10µl ab133518 - Anti-LRRK2 antibody [UDD3 30(12)] 2 x 10µl
FunctionPositively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). Plays a role in synaptic vesicle trafficking. Phosphorylates PRDX3. Has GTPase activity. May play a role in the phosphorylation of proteins central to Parkinson disease.
Tissue specificityExpressed in the brain. Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level). Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas.
Involvement in diseaseParkinson disease 8
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 12 LRR (leucine-rich) repeats.
Contains 1 protein kinase domain.
Contains 1 Roc domain.
Contains 7 WD repeats.
DomainThe seven-bladed WD repeat region is critical for synaptic vesicle trafficking and mediates interaction with multiple vesicle-associated presynaptic proteins.
The Roc domain mediates homodimerization and regulates kinase activity.
Cellular localizationMembrane. Cytoplasm. Perikaryon. Mitochondrion. Golgi apparatus. Cell projection, axon. Cell projection, dendrite. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Endosome. Lysosome. Mitochondrion outer membrane. Mitochondrion inner membrane. Mitochondrion matrix. Predominantly associated with intracytoplasmic vesicular and membranous structures (By similarity). Localized in the cytoplasm and associated with cellular membrane structures. Predominantly associated with the mitochondrial outer membrane of the mitochondria. Colocalized with RAB29 along tubular structures emerging from Golgi apparatus. Localizes in intracytoplasmic punctate structures of neuronal perikarya and dendritic and axonal processes.
- Information by UniProt
- augmented in rheumatoid arthritis 17
- Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993)
- Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)
- Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382)
- Anti-LRRK2 antibody [MJFF3 (c69-6)] - BSA and Azide free (ab183216)
- Alexa Fluor® 647 Anti-LRRK2 antibody [MJFF2 (c41-2)] (ab195023)
- Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] - Low endotoxin, Azide free (ab206035)
- Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] - BSA and Azide free (ab256581)
Immunofluorescent staining of SH-SY5Y cells fixed and permeablized with 4% PFA and 0.1% Triton X 100 using purified ab133518 at a dilution of 1/200. An Alexa Fluor® 488 goat anti-rabbit was used as the secondary (ab150077, 1/400) and the sample was stained with DAPI. The negative control is shown in bottom right hand panel - for the negative control, purified ab133518 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500.
Immunohistochemical staining of paraffin-embedded human kidney with purified ab133518 at a dilution of 1/100. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
ab133475, at 1/200 dilution, staining LRRK2 in paraffin embedded Human hippocampus tissue using immunohistochemical analysis.
Immunocytochemistry/Immunofluorescence analysis of A431 (Human epidermoid carcinoma cell line) cells labeling LRRK2 (phospho S935) with purified ab133450 at 1/100.
Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control: Secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).
ab133474 staining LRRK2 in Neuro-2a (mouse neuroblastoma cell line) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/500). ab150077 was used as the secondary antibody (1/1000). Tubulin stained using ab7291 (1/1000) and ab150120 (1/1000) as the secondary. Nuclear counter stained with DAPI.
Lane 1: Wild type A549 whole cell lysate (20 µg)
Lane 2: Wild type MEF whole cell lysate (20 µg)
Lane 3: LRRK2 knockout A549 whole cell lysate (20 µg)
Lane 4: LRRK2 knockout MEF whole cell lysate (20 µg)
ab133474 was shown to recognize LRRK2 in wild type A549 and MEF cells along with additional cross reative bands. Whilst signal was not seen in LRRK2 knockout cells. Wild-type and LRRK2 knockout samples were subjected to SDS-PAGE. Ab133474 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Wild-type and LRRK2 knockout MEF and A549 cells were provide as a generous gift from Professor Dario Alessi, MRC Protein Phosphorylation and Ubiquitination Unit (University of Dundee).
All lanes : Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181) at 1 µg/ml
Lane 1 : GFP-LRRK2 wt transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
Lane 2 : GFP-LRRK2 mutant D2017A transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
Lane 3 : GFP-LRRK2 mutant S1292A/G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
Lane 4 : GFP-LRRK2 mutant G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit (IRDye 800) at 1/10000 dilution
Predicted band size: 286 kDa
Observed band size: 313 kDa
Blocking/Dilution buffer: 5% BSA/TBST.
The image is provided by Dr. Jeremy Nicols, Parkinson’s Institute, Sunnyvale, CA, USA.
G2019S mutation results in an increased LRRK2 auto-phosphorylation including S1292 (lane 4).
Observed band size: 286 + 27 (GFP) = 313 kDa.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab263464 has not yet been referenced specifically in any publications.