Overview

  • Product name

    Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8]
    See all LRRK2 primary antibodies
  • Description

    Rabbit monoclonal [MJFR-19-7-8] to LRRK2 (phospho S1292)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human LRRK2 aa 1250-1350 (phospho S1292). The exact sequence is proprietary.
    Database link: Q5S007

  • Positive control

    • GFP-LRRK2 wt transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose; GFP-LRRK2 mutant G 2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose; LRRK2 wt transfected HEK293 lysate; LRRK2 mutant G2019S transfected HEK293 lysate.
  • General notes

    This antibody was developed with support from The Michael J. Fox Foundation.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    MJFR-19-7-8
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab203181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 286 kDa (predicted molecular weight: 286 kDa).

Target

  • Function

    Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). Plays a role in synaptic vesicle trafficking. Phosphorylates PRDX3. Has GTPase activity. May play a role in the phosphorylation of proteins central to Parkinson disease.
  • Tissue specificity

    Expressed in the brain. Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level). Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas.
  • Involvement in disease

    Parkinson disease 8
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 12 LRR (leucine-rich) repeats.
    Contains 1 protein kinase domain.
    Contains 1 Roc domain.
    Contains 7 WD repeats.
  • Domain

    The seven-bladed WD repeat region is critical for synaptic vesicle trafficking and mediates interaction with multiple vesicle-associated presynaptic proteins.
    The Roc domain mediates homodimerization and regulates kinase activity.
  • Post-translational
    modifications

    Autophosphorylated.
  • Cellular localization

    Membrane. Cytoplasm. Perikaryon. Mitochondrion. Golgi apparatus. Cell projection, axon. Cell projection, dendrite. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Endosome. Lysosome. Mitochondrion outer membrane. Mitochondrion inner membrane. Mitochondrion matrix. Predominantly associated with intracytoplasmic vesicular and membranous structures (By similarity). Localized in the cytoplasm and associated with cellular membrane structures. Predominantly associated with the mitochondrial outer membrane of the mitochondria. Colocalized with RAB29 along tubular structures emerging from Golgi apparatus. Localizes in intracytoplasmic punctate structures of neuronal perikarya and dendritic and axonal processes.
  • Information by UniProt
  • Database links

  • Alternative names

    • augmented in rheumatoid arthritis 17 antibody
    • AURA17 antibody
    • Dardarin antibody
    • Leucine rich repeat kinase 2 antibody
    • leucine rich repeat serine threonine protein kinase 2 antibody
    • Leucine-rich repeat serine/threonine-protein kinase 2 antibody
    • LRRK 2 antibody
    • LRRK2 antibody
    • LRRK2_HUMAN antibody
    • PARK 8 antibody
    • PARK8 antibody
    • RIPK7 antibody
    • ROCO 2 antibody
    • ROCO2 antibody
    see all

Images

  • All lanes : Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181) at 1 µg/ml

    Lane 1 : GFP-LRRK2 wt transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
    Lane 2 : GFP-LRRK2 mutant D2017A transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
    Lane 3 : GFP-LRRK2 mutant S1292A/G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
    Lane 4 : GFP-LRRK2 mutant G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit (IRDye 800) at 1/10000 dilution

    Predicted band size: 286 kDa
    Observed band size: 313 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% BSA/TBST.

    The image is provided by Dr. Jeremy Nicols, Parkinson’s Institute, Sunnyvale, CA, USA.

    G2019S mutation results in an increased LRRK2 auto-phosphorylation including S1292 (lane 4).

    Observed band size: 286 + 27 (GFP) = 313 kDa.

  • All lanes : Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181) at 1 µg/ml

    Lane 1 : LRRK2 mutant G2019S/S1292A transfected HEK293 lysate
    Lane 2 : LRRK2 mutant G2019S transfected HEK293 inhibitor treated lysate
    Lane 3 : LRRK2 mutant G2019S transfected HEK293 lysate
    Lane 4 : LRRK2 mutant S1292A transfected HEK293 lysate
    Lane 5 : LRRK2 wt transfected HEK293 inhibitor treated lysate
    Lane 6 : LRRK2 wt transfected HEK293 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2500 dilution

    Predicted band size: 286 kDa
    Observed band size: 286 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    The image is provided by Dr. Paul Davies, University of Dundee, Dundee Scotland, UK.

    G2019S mutation results in an increased LRRK2 auto-phosphorylation including S1292 (lane 3).

References

This product has been referenced in:

  • Kim J  et al. LRRK2 kinase plays a critical role in manganese-induced inflammation and apoptosis in microglia. PLoS One 14:e0210248 (2019). Read more (PubMed: 30645642) »
  • Ho DH  et al. G2019s LRRK2 promotes mitochondrial fission and increases TNFa-mediated neuroinflammation responses. Anim Cells Syst (Seoul) 23:106-111 (2019). Read more (PubMed: 30949397) »
See all 12 Publications for this product

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