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    lrrk2-phospho-s935-antibody-udd2-1012-ab133450.pdf

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RecombinantRabMAb

Recombinant Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)

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  • SDS
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Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)
  • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)
  • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)
  • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)
  • Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [UDD2 10(12)] to LRRK2 (phospho S935)
  • Suitable for: WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)]
    See all LRRK2 primary antibodies
  • Description

    Rabbit monoclonal [UDD2 10(12)] to LRRK2 (phospho S935)
  • Host species

    Rabbit
  • Specificity

    The antibody does not give a positive signal in U-87 MG, SH-SY-5Y and human fetal brain. Please contact our Scientific Support team if you have any question.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human LRRK2 aa 900-1000 (phospho S935). The exact sequence is proprietary.
    Database link: Q5S007
    (Peptide available as ab229694)

  • Positive control

    • WB: GFP LRRK2, GFP LRRK2 S910A, GFP LRRK2 S935A, LRRK2 WT MEF, LRRK2 WT MEF, Lymphoblastoid and RAW 264.7 cell lysates.
  • General notes

    This antibody was developed with the support of The Michael J. Fox Foundation (MJFF) and in partnership with Dr. Dario Alessi (MRC Protein Phosphorylation Unit, University of Dundee) to help accelerate LRRK2 research. Dr. Alessi has characterized several unique and high quality LRRK2 rabbit monoclonal antibodies, generated by Epitomics, to be made widely available for PD research community.

    LRRK2 (Leucine-rich repeat kinase 2, dardarin) is a multi-domain protein belonging to the ROCO family of proteins that contains a kinase and GTPase domain among its many protein interaction domains. LRRK2 is mutated in a significant number of Parkinson's disease (PD) patients. Mutations in this gene account for 4% of PD, and are observed in 1% of sporadic PD patients. The most common mutation replaces glycine 2019 with a serine that results in increased LRRK2 kinase activity. This indicates that inhibitors of LRRK2 kinase activity might be of therapeutic benefit for the treatment of Parkinson’s disease and has stimulated much activity in this field of research.
    Recent work has revealed that LRRK2 interacts with 14-3-3 phospho-binding adaptor isoforms that is mediated by phosphorylation of Ser910 and Ser935 located prior to the leucine rich repeat domain mediates. Interestingly, 14-3-3 binding has been linked to Parkinson’s disease as Ser910 as well as Ser935 and interaction with the 14-3-3 is inhibited by five of the six validated LRRK2 pathogenic mutations (R1441C, R1441G, R1441H, Y1699C and I2020T).

    The Dundee-MJFF LRRK2 PhosphoSer935 antibody will be of great utility in further understanding the link between 14-3-3 binding to LRRK2 and Parkinson’s disease as well as assessing the efficacy of LRRK2 inhibitors that are being developed. It should be noted the Dundee-MJFF antibody is highly selective and sensitive and can readily be used to monitor LRRK2 Ser935 phosphorylation in immunoblot analysis of 2-20 microgram amounts of whole cell extract. The Dundee-MJFF LRRK2 PhosphoSer935 recognizes both mouse and human endogenous LRRK2.

    A BSA and Azide free version of this product is available as ab172382.

    Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

    See other anti-rabbit secondary antibodies that can be used with this antibody.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This antibody was developed with support from The Michael J. Fox Foundation.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    UDD2 10(12)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Synuclein
    • Neuroscience
    • Diseases

Associated products

  • Alternative Versions

    • Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human LRRK2 (phospho S935) peptide (ab229694)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000. Detects a band of approximately 286 kDa (predicted molecular weight: 286 kDa).Can be blocked with Human LRRK2 (phospho S935) peptide (ab229694).
Notes
WB
1/1000. Detects a band of approximately 286 kDa (predicted molecular weight: 286 kDa).Can be blocked with Human LRRK2 (phospho S935) peptide (ab229694).
Application notes
Is unsuitable for ICC/IF or IHC-P.

Target

  • Function

    Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). Plays a role in synaptic vesicle trafficking. Phosphorylates PRDX3. Has GTPase activity. May play a role in the phosphorylation of proteins central to Parkinson disease.
  • Tissue specificity

    Expressed in the brain. Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level). Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas.
  • Involvement in disease

    Parkinson disease 8
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 12 LRR (leucine-rich) repeats.
    Contains 1 protein kinase domain.
    Contains 1 Roc domain.
    Contains 7 WD repeats.
  • Domain

    The seven-bladed WD repeat region is critical for synaptic vesicle trafficking and mediates interaction with multiple vesicle-associated presynaptic proteins.
    The Roc domain mediates homodimerization and regulates kinase activity.
  • Post-translational
    modifications

    Autophosphorylated.
  • Cellular localization

    Membrane. Cytoplasm. Perikaryon. Mitochondrion. Golgi apparatus. Cell projection, axon. Cell projection, dendrite. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Endosome. Lysosome. Mitochondrion outer membrane. Mitochondrion inner membrane. Mitochondrion matrix. Predominantly associated with intracytoplasmic vesicular and membranous structures (By similarity). Localized in the cytoplasm and associated with cellular membrane structures. Predominantly associated with the mitochondrial outer membrane of the mitochondria. Colocalized with RAB29 along tubular structures emerging from Golgi apparatus. Localizes in intracytoplasmic punctate structures of neuronal perikarya and dendritic and axonal processes.
  • Target information above from: UniProt accession Q5S007 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 120892 Human
    • Entrez Gene: 66725 Mouse
    • Omim: 609007 Human
    • SwissProt: Q5S007 Human
    • SwissProt: Q5S006 Mouse
    • Unigene: 187636 Human
    • Unigene: 37558 Mouse
    • Alternative names

      • augmented in rheumatoid arthritis 17 antibody
      • AURA17 antibody
      • Dardarin antibody
      • Leucine rich repeat kinase 2 antibody
      • leucine rich repeat serine threonine protein kinase 2 antibody
      • Leucine-rich repeat serine/threonine-protein kinase 2 antibody
      • LRRK 2 antibody
      • LRRK2 antibody
      • LRRK2_HUMAN antibody
      • PARK 8 antibody
      • PARK8 antibody
      • RIPK7 antibody
      • ROCO 2 antibody
      • ROCO2 antibody
      see all

    Images

    • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)
      Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)
      All lanes : Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450) at 1/1000 dilution (unpurified)

      Lane 1 : GFP LRRK2 lysate at 5 µg
      Lane 2 : GFP LRRK2 S910A lysate at 5 µg
      Lane 3 : GFP LRRK2 S935A lysate at 5 µg
      Lane 4 : LRRK2 WT MEF lysate at 20 µg
      Lane 5 : LRRK2 WT MEF lysate from LRRK2 IN1 treated cells
      at 20 µg
      Lane 6 : LRRK2 KO MEF lysate
      at 20 µg
      Lane 7 : LRRK2 KO MEF lysate from LRRK2 IN1 treated cells
      at 20 µg
      Lane 8 : Lymphoblastoid lysate
      at 30 µg
      Lane 9 : Lymphoblastoid lysate from LRRK2 IN1 treated cells
      at 30 µg

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 286 kDa

    • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)
      Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)Ito et al PLoS One. 2014 May 16;9(5):e97988. doi: 10.1371/journal.pone.0097988. eCollection 2014. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Inhibitor-induced dephosphorylation of kinase-inactive LRRK2.

      HEK-293 cells transfected with wild-type, or mutated LRRK2 (K1906A, K1906M, D1994A, D1994N, D2017A, S2032A or T2035A) were treated with 3 µM LRRK2-IN-1 (LRRK2 inhibitor) for 30 min. The phosphorylation of LRRK2 at Ser910, Ser935, or Ser955 was examined by immunoblotting. This showed that the responses of LRRK2 to the inhibitor varied among mutants.

      LRRK2 (phospho S935) was detected using ab133449.

      LRRK2 (phospho S935) was detected using ab133450. 

      LRRK2 (phospho S955) was detected using ab169521.

    • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)
      Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)
      All lanes : Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450) at 1/5000 dilution (purified)

      Lane 1 : WT-LRRK2 cell lysate - untreated
      Lane 2 : WT-LRRK2 cell lysate - treated with Lambda phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 286 kDa
      Observed band size: 286 kDa



      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)
      Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)
      Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450) at 1/1000 dilution (purified) + RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 286 kDa
      Observed band size: 286 kDa



      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    • Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)
      Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (51)

    Publishing research using ab133450? Please let us know so that we can cite the reference in this datasheet.

    ab133450 has been referenced in 51 publications.

    • De Miranda BR  et al. The industrial solvent trichloroethylene induces LRRK2 kinase activity and dopaminergic neurodegeneration in a rat model of Parkinson's disease. Neurobiol Dis 153:105312 (2021). PubMed: 33636387
    • Chen L  et al. LRRK2 inhibition potentiates PARP inhibitor cytotoxicity through inhibiting homologous recombination-mediated DNA double strand break repair. Clin Transl Med 11:e341 (2021). PubMed: 33784003
    • Wang X  et al. Understanding LRRK2 kinase activity in preclinical models and human subjects through quantitative analysis of LRRK2 and pT73 Rab10. Sci Rep 11:12900 (2021). PubMed: 34145320
    • Beilina A  et al. The Parkinson's Disease Protein LRRK2 Interacts with the GARP Complex to Promote Retrograde Transport to the trans-Golgi Network. Cell Rep 31:107614 (2020). PubMed: 32375042
    • Korecka JA  et al. Splice-Switching Antisense Oligonucleotides Reduce LRRK2 Kinase Activity in Human LRRK2 Transgenic Mice. Mol Ther Nucleic Acids 21:623-635 (2020). PubMed: 32736291
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    20 µg
    Specification
    HEK293
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 05 2021

    Western blot abreview for Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293FT cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Treatment
    NTC, LRRK2 siRNA, LRRK2 inhibitor (IN-1)
    Specification
    HEK293FT cells
    Blocking step
    Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 18 2020

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