Recombinant
RabMAb

Recombinant Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382)

Overview

  • Product name
    Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free
    See all LRRK2 primary antibodies
  • Description
    Rabbit monoclonal [UDD2 10(12)] to LRRK2 (phospho S935) - BSA and Azide free
  • Host species
    Rabbit
  • Specificity

    The antibody does not give a positive signal in U-87 MG, SH-SY-5Y and human fetal brain. Please contact our Scientific Support team if you have any question.

  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    within Human LRRK2 (phospho S935). The exact sequence is proprietary.
    Database link: Q5S007

  • Positive control
    • WB: GFP LRRK2, GFP LRRK2 S910A, GFP LRRK2 S935A, LRRK2 WT MEF, LRRK2 WT MEF, Lymphoblastoid and RAW 264.7 cell lysates. ICC/IF: A431 cells.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    Please view the parent abID of this product, ab133450, for information on validated applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This antibody was developed with support from The Michael J. Fox Foundation.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab172382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 286 kDa (predicted molecular weight: 286 kDa).

Please check the parent abID, ab133450, for a recommended dilution.

ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). Plays a role in synaptic vesicle trafficking. Phosphorylates PRDX3. Has GTPase activity. May play a role in the phosphorylation of proteins central to Parkinson disease.
    • Tissue specificity
      Expressed in the brain. Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level). Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas.
    • Involvement in disease
      Parkinson disease 8
    • Sequence similarities
      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 12 LRR (leucine-rich) repeats.
      Contains 1 protein kinase domain.
      Contains 1 Roc domain.
      Contains 7 WD repeats.
    • Domain
      The seven-bladed WD repeat region is critical for synaptic vesicle trafficking and mediates interaction with multiple vesicle-associated presynaptic proteins.
      The Roc domain mediates homodimerization and regulates kinase activity.
    • Post-translational
      modifications
      Autophosphorylated.
    • Cellular localization
      Membrane. Cytoplasm. Perikaryon. Mitochondrion. Golgi apparatus. Cell projection, axon. Cell projection, dendrite. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Endosome. Lysosome. Mitochondrion outer membrane. Mitochondrion inner membrane. Mitochondrion matrix. Predominantly associated with intracytoplasmic vesicular and membranous structures (By similarity). Localized in the cytoplasm and associated with cellular membrane structures. Predominantly associated with the mitochondrial outer membrane of the mitochondria. Colocalized with RAB29 along tubular structures emerging from Golgi apparatus. Localizes in intracytoplasmic punctate structures of neuronal perikarya and dendritic and axonal processes.
    • Information by UniProt
    • Database links
    • Alternative names
      • augmented in rheumatoid arthritis 17 antibody
      • AURA17 antibody
      • Dardarin antibody
      • Leucine rich repeat kinase 2 antibody
      • leucine rich repeat serine threonine protein kinase 2 antibody
      • Leucine-rich repeat serine/threonine-protein kinase 2 antibody
      • LRRK 2 antibody
      • LRRK2 antibody
      • LRRK2_HUMAN antibody
      • PARK 8 antibody
      • PARK8 antibody
      • RIPK7 antibody
      • ROCO 2 antibody
      • ROCO2 antibody
      see all

    Images

    • Inhibitor-induced dephosphorylation of kinase-inactive LRRK2.

      HEK-293 cells transfected with wild-type, K1906A, K1906M, D1994A, D1994N, D2017A, S2032A or T2035A LRRK2 were treated with (Panel A) 3 µM LRRK2-IN-1 or the solvent (0.1% DMSO) for 30 min.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133450).

    • Immunocytochemistry/Immunofluorescence analysis of A431 cells labelling LRRK2 (phospho S935) with purified ab133450 at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

      Control: primary antibody (1/100) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133450).

    • All lanes : Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450) at 1/1000 dilution

      Lane 1 : GFP LRRK2 lysate at 5 µg
      Lane 2 : GFP LRRK2 S910A lysate at 5 µg
      Lane 3 : GFP LRRK2 S935A lysate at 5 µg
      Lane 4 : LRRK2 WT MEF lysate at 20 µg
      Lane 5 : LRRK2 WT MEF lysate from LRRK2 IN1 treated cells at 20 µg
      Lane 6 : LRRK2 KO MEF lysate at 20 µg
      Lane 7 : LRRK2 KO MEF lysate from LRRK2 IN1 treated cells at 20 µg
      Lane 8 : Lymphoblastoid lysate at 30 µg
      Lane 9 : Lymphoblastoid lysate from LRRK2 IN1 treated cells at 30 µg

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 286 kDa



      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133450).

    • All lanes : Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450) at 1/5000 dilution

      Lane 1 : WT-LRRK2 cell lysate - untreated
      Lane 2 : WT-LRRK2 cell lysate - treated with Lambda phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 286 kDa



      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133450).

    • Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382) + NIH/3T3 (mouse embryo) whole cell lysates at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

      Predicted band size: 286 kDa


      Exposure time: 1 minute


      Blocking buffer and concentration: 5% NFDM/TBST

      Diluting buffer and concentration: 5% NFDM/TBST

    References

    This product has been referenced in:
    See all 10 Publications for this product

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