Overview

  • Product name

    Anti-LRRTM3 antibody
  • Description

    Rabbit polyclonal to LRRTM3
  • Host species

    Rabbit
  • Specificity

    This antibody will only detect the larger isoform of LRRTM3. Predicted to not cross-react with other LRRTM family members.
  • Tested applications

    Suitable for: ICC/IF, ICC, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    18 amino acid peptide from near to the carboxy terminus of Human LRRTM3 (NP_821079).

  • Positive control

    • Mouse brain tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab106640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
ICC Use a concentration of 2.5 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 66 kDa.
IHC-P Use at an assay dependent concentration.

Target

Images

  • Immunofluorescence of LRRTM3 in Human brain tissue with ab106640 at 20 ug/mL.
  • All lanes : Anti-LRRTM3 antibody (ab106640) at 0.5 µg/ml

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse brain tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 66 kDa

  • Immunohistochemistry of LRRTM3 in human brain tissue with ab106640 at 2.5 µg/ml.

References

ab106640 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting Abcam.

I am sorry to hear of the difficulties that you have been experiencing using these products and wish to thank you for the extensive testing that you have done. Of course we may refund or replace this product for you. Howeverwe will need some additional informations form you. Would you mind sending me the details of your protocol including such information as your sample preparations including denaturing and reducing steps, the amount of total protein per well, blocking procedures, antibody incubation steps, washes, images and any steps that you may have changes while troubleshooting.

I also encourage you to take advantage of our Abreview system when testing antibodies. It takes just a few minutes to leave a review and you can collect abpoints which you may redeem for products or Amazon gift cards while sharing information about the product with your collegues worldwide. To leave and Abreview, go to the webpafge for the product, click on the Abreview Tab right below the shipping information and clickhttps://www.abcam.com/index.html?pageconfig=your_reviews&intAbID=106640&strAccessMode=public_create. You will gain more Abpoints by submitting images as well.

Please let me know if you have any questions.

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Question

Protein from mouse and human brain was extracted using a lysis buffer containing 150mM NaCl, 50mM Tris pH 7.5 and 0.1% Triton.  Sonicated briefly and centrifuged to remove cell debris.   45ug of total protein was denatured in NuPage LDS sample buffer with 2-mercaptoethanol  and then heated to 85 degrees  before loading onto a NuPage 4-12% Bis- Tris Gel. The gel was run for 2hrs at 130mA.   Protein was transferred to Immobilon-P membrane and blocked with 5% milk TBST for one hour. The membrane was then probed with the LRRTM3 ab in 5%milk TBST overnight at a concentration of 1ug/ml The next morning the membrane was washed 3X 10 min wash in TBST buffer then probed for 1 hr with an Invitrogen Rabbit HRP conjugated secondary Ab in 5% milk TBST. Membranes were then washed 3X 10mins in TBST.   I have attached an image of the Western Blot and can provide a lighter exposure if required, but as you will see there is no difference between the WT and KO mouse brain tissue. This mouse KO mouse model has been published and we know from mRNA work that there should be no LRRTM3 expression.   Lanes 1         Wild type mouse brain lysate 2         Heterozgous LRRTM3 knockout brain lysate 3         Homozygous LRRTM3 knockout brain lysate 4         Wild type mouse brain lysate 5         Heterozgous LRRTM3 knockout brain lysate 6         Homozygous LRRTM3 knockout brain lysate 7         Human brain cerebellum 8         Human brain temporal cortex 9         HEK293T cell line overexpressing LRRTM3   I will certainly take a look at the Abreview page.   I look forward to hearing from you and please let me know if you need any further details.

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Answer

Thank you for contacting us. Your credit note ID is. I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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