For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
I was very interested in purchasing and testing your LRTM3 Ab. The Ab was used at a concentration of 1ug/ml. Please see the attached Western Blot of the following protein lysates; 1 Wild type mouse brain lysate 2 Heterozgous LRRTM3 knockout brain lysate 3 Homozygous LRRTM3 knockout brain lysate 4 Wild type mouse brain lysate 5 Heterozgous LRRTM3 knockout brain lysate 6 Homozygous LRRTM3 knockout brain lysate 7 Human brain cerebellum 8 Human brain temporal cortex 9 HEK293T cell line overexpressing LRRTM3 We are in a unique position of being able to test the specificity of this Ab by comparing WT to KO tissue and as you can see(sadly) from the blot there is absolutely no difference between the samples. I can provide a lighter exposure if needed.. On the data sheet your company claims that a 62Kd band is the endogenous mouse protein unfortunately this is not a specific band ….I wish it was. I would like to be refunded for this Ab. I can be contacted by phone or by email if you have any further questions. If in the future your company would be interested in testing a new LRRTM3 Ab please feel free to contact me as there are currently no good commercial antibodies for this gene.
Asked on Feb 10 2012
Thank you for contacting Abcam.
I am sorry to hear of the difficulties that you have been experiencing using these products and wish to thank you for the extensive testing that you have done. Of course we may refund or replace this product for you. Howeverwe will need some additional informations form you. Would you mind sending me the details of your protocol including such information as your sample preparations including denaturing and reducing steps, the amount of total protein per well, blocking procedures, antibody incubation steps, washes, images and any steps that you may have changes while troubleshooting.
I also encourage you to take advantage of our Abreview system when testing antibodies. It takes just a few minutes to leave a review and you can collect abpoints which you may redeem for products or Amazon gift cards while sharing information about the product with your collegues worldwide. To leave and Abreview, go to the webpafge for the product, click on the Abreview Tab right below the shipping information and clickhttps://www.abcam.com/index.html?pageconfig=your_reviews&intAbID=106640&strAccessMode=public_create. You will gain more Abpoints by submitting images as well.
Please let me know if you have any questions.
Answered on Feb 10 2012