Key features and details
- Rabbit polyclonal to LRSAM1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LRSAM1 antibody
See all LRSAM1 primary antibodies
DescriptionRabbit polyclonal to LRSAM1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- WB: LRSAM1 over-expression - HEK-293T whole cell lysate; IHC-P: Human cerebellum tissue.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 84 kDa.|
FunctionE3 ubiquitin-protein ligase that mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic (EGF receptors) and exocytic (HIV-1 viral proteins) cargos.
Tissue specificityHighly expressed in adult spinal cord motoneurons as well as in fetal spinal cord and muscle tissue.
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseCharcot-Marie-Tooth disease 2P (CMT2P) [MIM:614436]: An axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal degeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. Note=The disease is caused by mutations affecting the gene represented in this entry.
Sequence similaritiesContains 6 LRR (leucine-rich) repeats.
Contains 1 RING-type zinc finger.
Contains 1 SAM (sterile alpha motif) domain.
DomainThe coiled coil domains interact with the SB domain of TSG101.
The PTAP motifs mediate the binding to UEV domains.
Cellular localizationCytoplasm. Displays a punctuate distribution and localizes to a submembranal ring.
- Information by UniProt
- CMT2P antibody
- E3 ubiquitin protein ligase LRSAM1 antibody
- E3 ubiquitin-protein ligase LRSAM1 antibody
All lanes : Anti-LRSAM1 antibody (ab224433) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : LRSAM1 over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 84 kDa
Immunohistochemical analysis of human cerebellum tissue labeling LRSAM1 in the cytoplasm of Purkinje cells with ab224433 at 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224433 has not yet been referenced specifically in any publications.