Anti-LRSAM1 antibody (ab73113)
Key features and details
- Mouse polyclonal to LRSAM1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LRSAM1 antibody
See all LRSAM1 primary antibodies -
Description
Mouse polyclonal to LRSAM1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human LRSAM1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Human liver tissue lysate; LRSAM1-transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 84 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 84 kDa. |
Target
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Function
E3 ubiquitin-protein ligase that mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic (EGF receptors) and exocytic (HIV-1 viral proteins) cargos. -
Tissue specificity
Highly expressed in adult spinal cord motoneurons as well as in fetal spinal cord and muscle tissue. -
Pathway
Protein modification; protein ubiquitination. -
Involvement in disease
Charcot-Marie-Tooth disease 2P (CMT2P) [MIM:614436]: An axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal degeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. Note=The disease is caused by mutations affecting the gene represented in this entry. -
Sequence similarities
Contains 6 LRR (leucine-rich) repeats.
Contains 1 RING-type zinc finger.
Contains 1 SAM (sterile alpha motif) domain. -
Domain
The coiled coil domains interact with the SB domain of TSG101.
The PTAP motifs mediate the binding to UEV domains. -
Cellular localization
Cytoplasm. Displays a punctuate distribution and localizes to a submembranal ring. - Information by UniProt
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Database links
- Entrez Gene: 90678 Human
- Omim: 610933 Human
- SwissProt: Q6UWE0 Human
- Unigene: 495188 Human
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Alternative names
- CMT2P antibody
- E3 ubiquitin protein ligase LRSAM1 antibody
- E3 ubiquitin-protein ligase LRSAM1 antibody
see all
Images
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Anti-LRSAM1 antibody (ab73113) at 1/500 dilution + Human liver tissue lysate at 25 µg
Secondary
HRP-conjugated Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa -
All lanes : Anti-LRSAM1 antibody (ab73113) at 1/500 dilution
Lane 1 : LRSAM1-transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa
Datasheets and documents
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Datasheet download
References (8)
ab73113 has been referenced in 8 publications.
- Minaidou A et al. Deregulation of LRSAM1 expression impairs the levels of TSG101, UBE2N, VPS28, MDM2 and EGFR. PLoS One 14:e0211814 (2019). PubMed: 30726272
- Minaidou A et al. LRSAM1 Depletion Affects Neuroblastoma SH-SY5Y Cell Growth and Morphology: The LRSAM1 c.2047-1G>A Loss-of-Function Variant Fails to Rescue The Phenotype. Cell J 20:340-347 (2018). PubMed: 29845787
- Polajnar M et al. Expanding the host cell ubiquitylation machinery targeting cytosolic Salmonella. EMBO Rep 18:1572-1585 (2017). PubMed: 28784601
- Peeters K et al. Charcot-Marie-Tooth disease type 2G redefined by a novel mutation in LRSAM1. Ann Neurol 80:823-833 (2016). PubMed: 27686364
- Hu B et al. A novel missense mutation of CMT2P alters transcription machinery. Ann Neurol 80:834-845 (2016). PubMed: 27615052
- Bogdanik LP et al. Loss of the E3 ubiquitin ligase LRSAM1 sensitizes peripheral axons to degeneration in a mouse model of Charcot-Marie-Tooth disease. Dis Model Mech 6:780-92 (2013). WB . PubMed: 23519028
- Nicolaou P et al. A novel LRSAM1 mutation is associated with autosomal dominant axonal Charcot-Marie-Tooth disease. Eur J Hum Genet : (2012). Human . PubMed: 22781092
- Guernsey DL et al. Mutation in the gene encoding ubiquitin ligase LRSAM1 in patients with Charcot-Marie-Tooth disease. PLoS Genet 6: (2010). WB ; Human . PubMed: 20865121