Product nameAnti-LRWD1 antibody
See all LRWD1 primary antibodies
DescriptionRabbit polyclonal to LRWD1
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide, corresponding to a region between residues 432 and 482 of human LRWD1 (NP_690852.1)
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).|
FunctionAssociated component of the origin recognition complex (ORC) required to recruit and stabilize the ORC complex to chromatin. Probably required for the association of ORC on chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin: binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. It is unclear whether it recongnizes and binds these repressive marks by itself or needs additional factors.
Sequence similaritiesBelongs to the LRWD1 family.
Contains 3 LRR (leucine-rich) repeats.
Contains 5 WD repeats.
DomainThe WD repeats are required for the interaction with ORC components.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Chromosome > centromere. Chromosome > telomere. Localizes to heterochromatin during G1 phase. Restricted to centromeres or telomeres as cells progress though S phase. When cells enter mitosis, relocalizes to centromeres.
- Information by UniProt
- 1200011O22Rik antibody
- AU042569 antibody
- AW548074 antibody
All lanes : Anti-LRWD1 antibody (ab85738) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 71 kDa
Observed band size: 71 kDa
Additional bands at: 50 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
ab85738 has not yet been referenced specifically in any publications.