Overview

  • Product name

  • Description

    Rabbit polyclonal to LRWD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 432 and 482 of human LRWD1 (NP_690852.1)

  • Positive control

    • HeLa and 293T whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Associated component of the origin recognition complex (ORC) required to recruit and stabilize the ORC complex to chromatin. Probably required for the association of ORC on chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin: binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. It is unclear whether it recongnizes and binds these repressive marks by itself or needs additional factors.
    • Sequence similarities

      Belongs to the LRWD1 family.
      Contains 3 LRR (leucine-rich) repeats.
      Contains 5 WD repeats.
    • Domain

      The WD repeats are required for the interaction with ORC components.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus. Chromosome > centromere. Chromosome > telomere. Localizes to heterochromatin during G1 phase. Restricted to centromeres or telomeres as cells progress though S phase. When cells enter mitosis, relocalizes to centromeres.
    • Information by UniProt
    • Database links

    • Alternative names

      • 1200011O22Rik antibody
      • AU042569 antibody
      • AW548074 antibody
      • DKFZp434K1815 antibody
      • Leucine rich repeats and WD repeat domain containing 1 antibody
      • Leucine-rich repeat and WD repeat-containing protein 1 antibody
      • LRWD1 antibody
      • LRWD1_HUMAN antibody
      • ORC-associated protein antibody
      • ORCA antibody
      • Origin recognition complex-associated protein antibody
      see all

    Images

    • All lanes : Anti-LRWD1 antibody (ab85738) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Predicted band size: 71 kDa
      Observed band size: 71 kDa
      Additional bands at: 50 kDa, 80 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds

    References

    ab85738 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab85738.
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