Recombinant
RabMAb

Recombinant Anti-LSD2 / AOF1 antibody [EPR18508] (Alexa Fluor® 488) (ab223448)

Overview

  • Product name

    Anti-LSD2 / AOF1 antibody [EPR18508] (Alexa Fluor® 488)
    See all LSD2 / AOF1 primary antibodies
  • Description

    Rabbit monoclonal [EPR18508] to LSD2 / AOF1 (Alexa Fluor® 488)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human LSD2/ AOF1 aa 50-150. The exact sequence is proprietary.
    Database link: Q8NB78

  • Positive control

    • ICC/IF: HeLa cells. Flow Cyt: HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab223448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)

Flow Cyt 1/50.

Target

  • Function

    Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4.
  • Sequence similarities

    Belongs to the flavin monoamine oxidase family.
    Contains 1 CW-type zinc finger.
    Contains 1 SWIRM domain.
  • Domain

    The SWIRM domain may act as an anchor site for a histone tail.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Form

    There are 3 isoforms produced by alternative splicing.
  • Alternative names

    • amine oxidase (flavin containing) domain 1 antibody
    • amine oxidase, flavin containing 1 antibody
    • AOF1 antibody
    • bA204B7.3 antibody
    • C6orf193 antibody
    • dJ298J15.2 antibody
    • Flavin-containing amine oxidase domain-containing protein 1 antibody
    • FLJ33898 antibody
    • FLJ34109 antibody
    • FLJ43328 antibody
    • KDM1B antibody
    • KDM1B_HUMAN antibody
    • LSD2 antibody
    • lysine (K)-specific demethylase 1B antibody
    • Lysine-specific demethylase 2 antibody
    • Lysine-specific histone demethylase 1B antibody
    • Lysine-specific histone demethylase 2 antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab223448 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223448, 1/50 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

  • ab223448 staining LSD2 / AOF1 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223448 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab223448 has not yet been referenced specifically in any publications.

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