Key features and details
- Rabbit polyclonal to LSM1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LSM1 antibody
See all LSM1 primary antibodies
DescriptionRabbit polyclonal to LSM1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis
Recombinant fragment within Human LSM1 (internal sequence). The exact sequence is proprietary.
Database link: O15116
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extracts.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/100 - 1/500.|
|WB||1/500 - 1/3000. Predicted molecular weight: 15 kDa.|
FunctionPlays a role in replication-dependent histone mRNA degradation. Binds specifically to the 3'-terminal U-tract of U6 snRNA.
Tissue specificityHas elevated expression in pancreatic cancer and in several cancer-derived cell lines.
Sequence similaritiesBelongs to the snRNP Sm proteins family.
- Information by UniProt
- Cancer associated Sm like protein antibody
- Cancer associated Sm-like antibody
- Cancer-associated Sm-like antibody
All lanes : Anti-LSM1 antibody (ab229316) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell extracts
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 15 kDa
LSM1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg ab229316. Western blot was performed from the immunoprecipitate using ab229316.
Lane 1: HEK-293T whole cell extract (Input).
Lane 2: Control IgG IP in HEK-293T whole cell extract.
Lane 3: ab229316 IP in HEK-293T whole cell extract.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229316 has not yet been referenced specifically in any publications.