Recombinant Anti-LSM10 antibody [EPR13843] (ab180128)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13843] to LSM10
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-LSM10 antibody [EPR13843]
See all LSM10 primary antibodies -
Description
Rabbit monoclonal [EPR13843] to LSM10 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human LSM10 aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: Q969L4 -
Positive control
- Jurkat and HepG2 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13843 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab180128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP | 1/40. | |
WB | 1/5000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 14 kDa). |
Target
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Relevance
LSM10 is a 14 kDa polypeptide that is a member of the Sm/Lsm protein family. Like U7 snRNA, LSM10 is enriched in Cajal or coiled bodies (CBs) in the nucleus. LSM10 associates with the U7 snRNP and this interaction is dictated by the special Sm binding site of U7 snRNA. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 84967 Human
- Entrez Gene: 116748 Mouse
- Entrez Gene: 366468 Rat
- SwissProt: Q969L4 Human
- SwissProt: Q8QZX5 Mouse
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Alternative names
- LSM 10 antibody
- LSM10 U7 small nuclear RNA associated antibody
- MGC15749 antibody
see all
Images
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Western blot analysis of immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab180128 at 1/40 dilution. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
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All lanes : Anti-LSM10 antibody [EPR13843] (ab180128) at 1/5000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 14 kDa
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab180128 has not yet been referenced specifically in any publications.