Anti-LSM11 antibody [OTI2G7] (ab236396)
Key features and details
- Mouse monoclonal [OTI2G7] to LSM11
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-LSM11 antibody [OTI2G7]
See all LSM11 primary antibodies -
Description
Mouse monoclonal [OTI2G7] to LSM11 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human LSM11 aa 1 to the C-terminus. Produces in E.coli (NP_775762).
Database link: P83369 -
Positive control
- WB: pCMV6-ENTRY LSM11 transfected HEK-293T cell lysate. IHC-P: Human prostate carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI2G7 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Predicted molecular weight: 40 kDa.
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IHC-P |
1/2000.
Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 minutes. |
Notes |
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WB
1/2000. Predicted molecular weight: 40 kDa. |
IHC-P
1/2000. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 minutes. |
Target
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Relevance
LSM11 is a 39.5 kDa protein that is a component of a specialized SMN complex also containing Lsm10. Lsm11 not only specifies the assembly of the U7 Sm core but also fulfills an important role in U7 snRNP-mediated histone mRNA processing. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 134353 Human
- SwissProt: P83369 Human
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Alternative names
- 2210404M20Rik antibody
- LSM11 homolog, U7 small nuclear RNA associated antibody
- LSM11, U7 small nuclear RNA associated antibody
- U7 snRNA-associated Sm-like protein LSm11 antibody
Images
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Paraffin-embedded human prostate carcinoma tissue stained for LSM11 using ab236396 at 1/2000 in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM TRis buffer (pH 8.5) at 120°C for 3 minutes.
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All lanes : Anti-LSM11 antibody [OTI2G7] (ab236396) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY LSM11 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 40 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236396 has not yet been referenced specifically in any publications.