Overview

  • Product name
    Anti-LSM12 antibody [EPR12282-87]
    See all LSM12 primary antibodies
  • Description
    Rabbit monoclonal [EPR12282-87] to LSM12
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IP, WBmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human LSM12. The exact sequence is proprietary.
    Database link: Q3MHD2

  • Positive control
    • 293T, HepG2, HeLa and fetal brain lysates; Human endometrial carcinoma tissue; HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12282-87
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab173292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IHC-P 1/50 - 1/100.
IP 1/10 - 1/100.
WB 1/10000 - 1/50000. Predicted molecular weight: 22 kDa.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    Images

    • All lanes : Anti-LSM12 antibody [EPR12282-87] (ab173292) at 1/10000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 22 kDa

    • Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling LSM12 with ab173292 at 1/50 dilution.

    • Immunofluorescent analysis of HepG2 cells labeling LSM12 with ab173292 at 1/100 dilution.

    • Western blot analysis on immunoprecipitation pellet from 1) HeLa cell lysate or 2) 1X PBS using ab173292 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    This product has been referenced in:
    • Ayache J  et al. P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes. Mol Biol Cell 26:2579-95 (2015). WB ; Human . Read more (PubMed: 25995375) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab173292.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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