Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12282] to LSM12
- Suitable for: Flow Cyt, WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-LSM12 antibody [EPR12282]
See all LSM12 primary antibodies
DescriptionRabbit monoclonal [EPR12282] to LSM12
Tested applicationsSuitable for: Flow Cyt, WB, ICC/IF, IPmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human LSM12 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q3MHD2
- 293T, HepG2 and HeLa whole cell lysate (ab150035); HepG2 and 293T cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab173291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/5000. Predicted molecular weight: 22 kDa.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
RelevanceLSM12 belongs to the LSM12 family. The exact function of LSM12 is not known.
- FLJ30656 antibody
- LSM12 homolog antibody
- LSM12 homolog (S. cerevisiae) antibody
All lanes : Anti-LSM12 antibody [EPR12282] (ab173291) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 22 kDa
Immunofluorescent analysis of HepG2 cells labeling LSM12 with ab173291 at 1/100 dilution.
Flow cytometry analysis of permeabilized 293T cells labeling LSM12 with ab173291 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).
Western blot analysis on immunoprecipitation pellet from 1) HepG2 cell lysate or 2) 1X PBS (negative control) using ab173291 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab173291 has not yet been referenced specifically in any publications.