Overview

  • Product name

    Anti-LSM12 antibody [EPR12282]
    See all LSM12 primary antibodies
  • Description

    Rabbit monoclonal [EPR12282] to LSM12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IF, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human LSM12 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q3MHD2

  • Positive control

    • 293T, HepG2 and HeLa whole cell lysate (ab150035); HepG2 and 293T cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12282
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab173291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    Images

    • All lanes : Anti-LSM12 antibody [EPR12282] (ab173291) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 22 kDa

    • Immunofluorescent analysis of HepG2 cells labeling LSM12 with ab173291 at 1/100 dilution.

    • Flow cytometry analysis of permeabilized 293T cells labeling LSM12 with ab173291 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).

    • Western blot analysis on immunoprecipitation pellet from 1) HepG2 cell lysate or 2) 1X PBS (negative control) using ab173291 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    ab173291 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab173291.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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