Overview

  • Product name

    Anti-LSM5 antibody [EPR10690]
    See all LSM5 primary antibodies
  • Description

    Rabbit monoclonal [EPR10690] to LSM5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human LSM5 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Y4Y9

  • Positive control

    • T47-D lysate, Human astrocytoma or stomach tissue, T47-D cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 10 kDa.
IHC-P 1/50 - 1/100.
Flow Cyt 1/110.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • Flow cytometry analysis of T47-D cells using ab184568 at a 1/110 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

  • Immunofluorescence analysis of 4% paraformaldehyde fixed T47-D cells labeling LSM5 with ab184568 at a 1/500 dilution. Goat anti rabbit IgG (Alexa Fluor®555) secondary used at a 1/200 diution. Conterstained with Dapi (right image).

  • Immunohistochemical analysis of paraffin embedded Human stomach tissue labeling LSM5 with ab184568 a 1/100 dilution. Counterstained with Hematoxylin.

  • Immunohistochemical analysis of paraffin embedded Human astrocytoma tissue labeling LSM5 with ab184568 at a 1/100 dilution. Counterstained with Hematoxylin.

  • Anti-LSM5 antibody [EPR10690] (ab184568) at 1/10000 dilution + T47-D lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 10 kDa

References

ab184568 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab184568.
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