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Recombinant full length protein corresponding to Human LSMD1 aa 2-173. Full length chain of isoform 2
MAVAVGVRAAPVLGLARALVLGLRGSQAARWRGWGTAAPGSLWALCECPA GCRELWFRGRAAWAARSERLLHPAQDSDGEREDSAAERARQQLEALLNKT MRIRMTDGRTLVGCFLCTDRDCNVILGSAQEFLKPSDSFSAGEPRVLGLA MVPGHHIVSIEVQRESLTGPPYL
Our Abpromise guarantee covers the use of ab236965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000.|
|IHC-P||1/100 - 1/500.|
|ICC/IF||1/50 - 1/500.|
PC-3 (human prostate adenocarcinoma cell line) cells stained for LSMD1 (Green) using ab236965 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.
Paraffin-embedded human kidney tissue stained for LSMD1 using ab236965 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
ab236965 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"