Anti-LSP1 antibody (ab21637)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-LSP1 antibody
See all LSP1 primary antibodies -
DescriptionRabbit polyclonal to LSP1
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Host speciesRabbit
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SpecificityThis epitope affinity purified rabbit polyclonal antibody reacts predominantly with LSP1. By immunoblot analysis, a ~52 kDa immunoreactive protein from Mouse Spleen tissue lysate was detected (sample were boiled prior to subject 10% SDSpolyacrylamide gel electrophoresis). This band is abolished by the depletion of the synthetic immunizing peptide.
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Tested applicationsSuitable for: ELISA, IP, WBmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide:
KLARQASIELP
, corresponding to amino acids 246-256 of Human LSP1.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Proclin 300
Constituents: PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab21637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use a concentration of 0.1 - 1 µg/ml. | |
| IP | Use 3µg for 105 cells. | |
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 52 kDa. |
Target
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FunctionMay play a role in mediating neutrophil activation and chemotaxis.
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Tissue specificityActivated T-lymphocytes.
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Post-translational
modificationsPhosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis. -
Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 4046 Human
- Entrez Gene: 16985 Mouse
- Omim: 153432 Human
- SwissProt: P33241 Human
- SwissProt: P19973 Mouse
- Unigene: 56729 Human
- Unigene: 234003 Mouse
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Alternative names
- 47 kDa actin binding protein antibody
- 47 kDa actin-binding protein antibody
- 52 kDa phosphoprotein antibody
see all
References
This product has been referenced in:
- Wu JL et al. Temporal regulation of Lsp1 O-GlcNAcylation and phosphorylation during apoptosis of activated B cells. Nat Commun 7:12526 (2016). Read more (PubMed: 27555448) »
